All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature

被引:23
作者
Bechert, T
Heck, S
Fleig, U
Diekmann, S
Hegemann, JH
机构
[1] Inst Mol Biotechnol, Mol Biol Abt, D-07708 Jena, Germany
[2] Univ Giessen, Inst Mikrobiol & Mol Biol, D-35392 Giessen, Germany
关键词
D O I
10.1093/nar/27.6.1444
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
All 16 centromere DNA regions of Saccharomyces cerevisiae including 90 bp framing sequences on either side were cloned, These 300 bp long centromere regions were analysed by native polyacrylamide gel electrophoresis and found to display a reduced mobility indicative of DNA curvature, The degree of curvature is centromere dependent. The experimental data were confirmed by computer analysis of the 3-dimensional structure of the CEN DNAs, Altogether these data provide further evidence for a model for budding yeast centromeres in which CEN DNA structure could be important for the assembly, activity and/or regulation of the centromere protein-DNA complex.
引用
收藏
页码:1444 / 1449
页数:6
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