Comparative evaluation of two commercial assays for direct detection of Mycobacterium tuberculosis in respiratory specimens

被引:16
作者
Gamboa, F
Manterola, JM
Lonca, J
Matas, L
Cardona, PJ
Padilla, E
Viñado, B
Domínguez, J
Hernández, A
Ausina, V
机构
[1] Hosp Univ Germans Trias & Pujol, Microbiol Serv, E-8916 Barcelona, Spain
[2] Univ Autonoma Barcelona, Fac Med, Dept Genet & Microbiol, E-08193 Barcelona, Spain
[3] Pontificia Univ Javeriana, Fac Ciencias, Dept Microbiol, Unidad Microbiol Especial, Bogota, Colombia
关键词
Mycobacterium tuberculosis; detection; respiratory specimens;
D O I
10.1007/BF01691110
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Two commercial systems for the amplification and detection of Mycobacterium tuberculosis directly from respiratory samples were compared. The Roche Cobas Amplicor MTB Test and the Roche manual Amplicor MTB Test (Roche Diagnostic Systems, USA) were applied to 755 decontaminated respiratory specimens collected from 470 patients. Results were compared with those of acid-fast staining and culture. A total of 251 specimens were collected from 156 patients diagnosed with pulmonary tuberculosis, including 28 specimens corresponding to 13 patients that were receiving antituberculous treatment. Given the overall positivity rate of 33.2% (251/755), the sensitivity, specificity, and positive and negative predictive values were 92.4, 100, 100, and 96.5%, respectively, for the Cobas Amplicor MTB Test and 90.8, 100, 100, and 95.8%, respectively, for the Amplicor MTB Test. For 204 (81.3%) smear positive specimens and 47 (19.7%) smear negative specimens, the sensitivity values were 100 and 59.6%, respectively, for the Cobas Amplicor MTB Test and 100 and 51%, respectively, for the Amplicor MTB Test. There were no statistically significant differences in sensitivity or specificity between the two assays and culture (p>0.05). The overall results of both assays were concordant for 99.5% of the samples. It is concluded that although both nucleic acid amplification methods are rapid and specific for the detection of Mycobacterium tuberculosis complex in respiratory specimens, the Cobas Amplicor MTB Test appears to be slightly more sensitive than the Amplicor MTB Test when smear negative specimens are investigated.
引用
收藏
页码:151 / 157
页数:7
相关论文
共 30 条
  • [11] DIRECT DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL SPECIMENS BY DNA AMPLIFICATION
    DEWIT, D
    STEYN, L
    SHOEMAKER, S
    SOGIN, M
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (11) : 2437 - 2441
  • [12] SIMPLE METHOD FOR PRODUCTION OF INTERNAL CONTROL DNA FOR MYCOBACTERIUM-TUBERCULOSIS POLYMERASE CHAIN-REACTION ASSAYS
    DEWIT, D
    WOOTTON, M
    ALLAN, B
    STEYN, L
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (08) : 2204 - 2207
  • [13] Detection of Mycobacterium tuberculosis in respiratory specimens by strand displacement amplification of DNA
    Down, JA
    OConnell, MA
    Dey, MS
    Walters, AH
    Howard, DR
    Little, MC
    Keating, WE
    Zwadyk, P
    Haaland, PD
    McLaurin, DA
    Cole, G
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (04) : 860 - 865
  • [14] DETECTION OF MYCOBACTERIUM-TUBERCULOSIS DNA IN CLINICAL-SAMPLES BY USING A SIMPLE LYSIS METHOD AND POLYMERASE CHAIN-REACTION
    FOLGUEIRA, L
    DELGADO, R
    PALENQUE, E
    NORIEGA, AR
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (04) : 1019 - 1021
  • [15] EVALUATION OF ACRIDINIUM-ESTER-LABELED DNA PROBES FOR IDENTIFICATION OF MYCOBACTERIUM-TUBERCULOSIS AND MYCOBACTERIUM-AVIUM-MYCOBACTERIUM-INTRACELLULARE COMPLEX IN CULTURE
    GOTO, M
    OKA, S
    OKUZUMI, K
    KIMURA, S
    SHIMADA, K
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (11) : 2473 - 2476
  • [16] Heifets Leonid B., 1994, P85
  • [17] IMPACT OF HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION ON THE EPIDEMIOLOGY, CLINICAL-FEATURES, MANAGEMENT, AND CONTROL OF TUBERCULOSIS
    HOPEWELL, PC
    [J]. CLINICAL INFECTIOUS DISEASES, 1992, 15 (03) : 540 - 547
  • [18] Evaluation of automated COBAS AMPLICOR PCR system for detection of several infectious agents and its impact on laboratory management
    Jungkind, D
    DiRenzo, S
    Beavis, KG
    Silverman, NS
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (11) : 2778 - 2783
  • [19] MORE RELIABLE PCR FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL-SAMPLES
    KOX, LFF
    RHIENTHONG, D
    MIRANDA, AM
    UDOMSANTISUK, N
    ELLIS, K
    VANLEEUWEN, J
    VANHEUSDEN, S
    KUIJPER, S
    KOLK, AHJ
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (03) : 672 - 678
  • [20] EVALUATION OF PRACTICAL CHROMATOGRAPHIC PROCEDURES FOR IDENTIFICATION OF CLINICAL ISOLATES OF MYCOBACTERIA
    LUQUIN, M
    AUSINA, V
    CALAHORRA, FL
    BELDA, F
    BARCELO, MG
    CELMA, C
    PRATS, G
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (01) : 120 - 130