Overexpression of VvWRKY2 in tobacco enhances broad resistance to necrotrophic fungal pathogens

被引:100
作者
Mzid, Rim
Marchive, Chloe
Blancard, Dominique
Deluc, Laurent
Barrieu, Francois
Corio-Costet, Marie-France
Drira, Nourredine
Hamdi, Said
Lauvergeat, Virginie
机构
[1] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
[2] Univ Nevada, Dept Biochem & Mol Biol, Reno, NV 89557 USA
[3] Univ Bordeaux 1, Univ Bordeaux 2, INRA, Unite Mixte Rech 1287,Inst Sci Vigne Vin, F-33883 Villenave Dornon, France
[4] Fac Sci, Lab Biotechnol Vegetales Appl Ameliorat Cultures, Sfax, Tunisia
[5] INRA, Ecole Natl Ingn Travaux Agricoles, Unit Mixte Rech Sante Vegetale, F-33883 Villenave Dornon, France
关键词
D O I
10.1111/j.1399-3054.2007.00975.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
WRKY genes encode proteins belonging to a large family of transcription factors that are involved in various developmental and physiological processes and in plant responses to pathogen infections. In the present work, a full-length cDNA from a Vitis vinifera L. cv. Cabernet Sauvignon grape berry library was isolated and characterized. The cDNA, designated VvWRKY2, encodes a polypeptide of 536 amino acids that shows the structural features of group I of WRKY protein family. VvWRKY2 is expressed in the different organs of healthy grapevine plants. In leaves, VvWRKY2 is induced by wounding and after infection with Plasmopara viticola. Constitutive expression of VvWRKY2 in tobacco reduced the susceptibility of transgenic tobacco to three types of fungal pathogens infecting different parts of the plant: Botrytis cinerea (leaves), Pythium spp. (roots) and Alternaria tenuis (seeds). The results indicate that VvWRKY2 may be involved in the resistance of grapevine against the pathogens.
引用
收藏
页码:434 / 447
页数:14
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