CSF-enriched antibodies do not share specificities among MS patients

被引：15

作者：

Cortese, I

Capone, S

Luchetti, S

Grimaldi, LME

Nicosia, A

Cortese, R

机构：

[1] Ist Ric Biol Mol P Angeletti, I-00040 Pomezia, Roma, Italy

[2] Univ Rome, Neurol Clin, I-00100 Rome, Italy

[3] IRCCS, I-00173 Rome, Italy

[4] Univ Milano, IRCCS, Osped S Raffaele, Dept Neurol,Neuroimmunol Unit, I-20132 Milan, Italy

来源：

MULTIPLE SCLEROSIS | 1998年 / 4卷 / 03期

关键词：

multiple sclerosis; CSF; oligoclonal bonds; phage library;

D O I：

10.1191/135245898678909268

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

The specificity of the oligoclonal immunoglobulins found in MS CSF is unknown. We have previously shown that random peptide libraries displayed on phage can be used to identify specific ligands for CSF antibodies. Here we describe the use of this tool in the attempt to identify MS-specific CSF-enriched antibody reactivities with potential pathogenic diagnostic or Prognostic value. Applying different experimental strategies several ligands reacting with CSF-enriched antibodies were identified. When tested against a panel of 55 MS patients, none of the ligands found were recognized by antibodies shored by any two patients. We used the selected peptides to demonstrate the stability in time of CSF-enriched antibodies notwithstanding disease progression.

引用

页码：118 / 123

页数：6

共 18 条

[1] Identification of peptides specific for cerebrospinal fluid antibodies in multiple sclerosis by using phage libraries [J].