Capillary isoelectric focusing with UV-induced fluorescence detection

被引：34

作者：

Horká, M

Willimann, T

Blum, M

Nording, P

Friedl, Z

Slais, K

机构：

[1] Acad Sci Czech Republ, Inst Analyt Chem, CS-61142 Brno, Czech Republic

[2] Fluka Prod GmbH, CH-9471 Buchs SG, Switzerland

[3] Brno Univ Technol, Fac Chem, Brno 61200, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 916卷 / 1-2期

关键词：

isoelectric focusing; detection; electrophoresis; fluorescence detection; proteins;

D O I：

10.1016/S0021-9673(00)01069-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Low-molecular-mass fluorescent compounds excitable in the near UV region with suitable acidobasic and electrophoretic properties are suggested as isoelectric point (pI) markers for isoelectric focusing (IEF) with UV photometric and UV excited fluorometric detection. The experimental set-up of capillary IEF with W excited fluorometric detection and properties of new UV-induced fluorescent pi markers are given. The pI values of 18 new pI markers determined independently of IEF methods range from 2.1 to 10.3. The examples of separation of new pi markers together with derivatized proteins by capillary IEF with photometric or fluorometric detection are presented. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：65 / 71

页数：7