Production of infectious human papillomavirus independently of viral replication and epithelial cell differentiation

被引:90
作者
Pyeon, D
Lambert, PF
Ahlquist, P
机构
[1] Univ Wisconsin, Inst Mol Virol, Madison, WI 53706 USA
[2] Univ Wisconsin, McArdle Lab Canc Res, Madison, WI 53706 USA
[3] Univ Wisconsin, Howard Hughes Med Inst, Madison, WI 53706 USA
关键词
infection; virions; virus packaging; capsid; DNA encapsidation;
D O I
10.1073/pnas.0504020102
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Papillomavi ruses are small DNA viruses that are associated with benign and malignant epithelial lesions, including > 95 % of cervical cancers and approximate to 20 % of head and neck cancers. Because papillomavirus replication and virion production are tied to epithelial cell differentiation, infectious papillomavirus virion production has been limited to cumbersome organotypic cultures and mouse xenografts. Consequent difficulties in obtaining useful amounts of wild-type or mutant human papillomavirus (HPV) virions have greatly limited studies on many aspects of papillomavirus biology. To overcome these limitations, we developed a system to encapsidate the full-length papillomaviral genome into infectious virions, independently of viral DNA replication and epithelial differentiation. This transient-transfection-based system produces > 1,000 times more infectious virus per cell culture dish than the much more labor-intensive organotypic culture. Furthermore, we show that this method allows the facile generation of infectious particles containing wild-type, mutant, or chimeric papillomaviral genomes, overcoming barriers to studying many facets of replication, host interactions, and vaccine and drug development, which has been limited by the insufficient availability of infectious virions.
引用
收藏
页码:9311 / 9316
页数:6
相关论文
共 48 条
  • [1] Factors in heteromorphosis in planarians.
    Bardeen, CR
    [J]. ARCHIV FUR ENTWICKLUNGSMECHANIK DER ORGANISMEN, 1903, 16 (01): : 1 - 20
  • [2] The human papillomavirus E7 oncoprotein abrogates signaling mediated by interferon-α
    Barnard, P
    McMillan, NAJ
    [J]. VIROLOGY, 1999, 259 (02) : 305 - 313
  • [3] Isolation and propagation of human papillomavirus type 16 in human xenografts implanted in the severe combined immunodeficiency mouse
    Bonnez, W
    Darin, C
    Borkhuis, C
    Jensen, KM
    Reichman, RC
    Rose, RC
    [J]. JOURNAL OF VIROLOGY, 1998, 72 (06) : 5256 - 5261
  • [4] E7-specific cytotoxic T cell tolerance in HPV-transgenic mice
    Borchers, A
    Braspenning, J
    Meijer, J
    Osen, W
    Gissmann, L
    Jochmus, I
    [J]. ARCHIVES OF VIROLOGY, 1999, 144 (08) : 1539 - 1556
  • [5] NORMAL KERATINIZATION IN A SPONTANEOUSLY IMMORTALIZED ANEUPLOID HUMAN KERATINOCYTE CELL-LINE
    BOUKAMP, P
    PETRUSSEVSKA, RT
    BREITKREUTZ, D
    HORNUNG, J
    MARKHAM, A
    FUSENIG, NE
    [J]. JOURNAL OF CELL BIOLOGY, 1988, 106 (03) : 761 - 771
  • [6] EPITHELIAL DIFFERENTIATION FAILS TO SUPPORT REPLICATION OF CLONED HUMAN PAPILLOMAVIRUS TYPE-16 DNA IN TRANSFECTED KERATINOCYTES
    BRUNE, W
    DURST, M
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1995, 104 (02) : 277 - 281
  • [7] Maturation of papillomavirus capsids
    Buck, CB
    Thompson, CD
    Pang, YYS
    Lowy, DR
    Schiller, JT
    [J]. JOURNAL OF VIROLOGY, 2005, 79 (05) : 2839 - 2846
  • [8] Efficient intracellular assembly of papillomaviral vectors
    Buck, CB
    Pastrana, DV
    Lowy, DR
    Schiller, JT
    [J]. JOURNAL OF VIROLOGY, 2004, 78 (02) : 751 - 757
  • [9] MACROPHAGE-TROPIC HUMAN-IMMUNODEFICIENCY-VIRUS ISOLATES FROM DIFFERENT PATIENTS EXHIBIT UNUSUAL V3 ENVELOPE SEQUENCE HOMOGENEITY IN COMPARISON WITH T-CELL-TROPIC ISOLATES - DEFINITION OF CRITICAL AMINO-ACIDS INVOLVED IN CELL TROPISM
    CHESEBRO, B
    WEHRLY, K
    NISHIO, J
    PERRYMAN, S
    [J]. JOURNAL OF VIROLOGY, 1992, 66 (11) : 6547 - 6554
  • [10] Surface conformational and linear epitopes on HPV-16 and HPV-18 L1 virus-like particles as defined by monoclonal antibodies
    Christensen, ND
    Dillner, J
    Eklund, C
    Carter, JJ
    Wipf, GC
    Reed, CA
    Cladel, NM
    Galloway, DA
    [J]. VIROLOGY, 1996, 223 (01) : 174 - 184