Plaque reduction neutralization test for measles antibodies: Description of a standardised laboratory method for use in immunogenicity studies of aerosol vaccination

被引：159

作者：

Cohen, Bernard J. ^{[1
]}

Audet, Susette ^{[2
]}

Andrews, Nick ^{[1
]}

Beeler, Judy ^{[2
]}

机构：

[1] Hlth Protect Agcy, Ctr Infect, London NW9 5EQ, England

[2] US FDA, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA

来源：

VACCINE | 2007年 / 26卷 / 01期

关键词：

measles; plaque reduction neutralization test;

D O I：

10.1016/j.vaccine.2007.10.046

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background: Clinical trials of measles vaccination administered as aerosol are planned with the aim of obtaining Licensure. Measles antibody Levels wilt be measured using the plaque reduction neutralization test (PRNT) to assess antibody responses as a surrogate marker of efficacy. Methods: A working group examined laboratory protocols for measles PRNT in use at three reference centres and agreed to a standardised procedure, which was subsequently validated. Results: Assay validation showed quantitative results varied approximately threefold both within and between assays. The lower limit of detection was approximately 20 milliInternational Units/mL.

引用

页码：59 / 66

页数：8

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