The MGEA6 multigene family has an active locus on 14q and at least nine pseudogenes on different chromosomes

被引：11

作者：

Comtesse, N ^{[1
]}

Reus, K ^{[1
]}

Meese, E ^{[1
]}

机构：

[1] Univ Saarlandes Kliniken, Inst Human Genet, D-64421 Homburg, Germany

来源：

GENOMICS | 2001年 / 75卷 / 1-3期

关键词：

multigene family; pseudogene; retrotransposition; meningioma; MGEA6;

D O I：

10.1006/geno.2001.6576

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The meningioma expressed antigen-6 (MGEA6) was originally identified as an immunogenic antigen in meningioma patients. Somatic hybrid panel mapping and fluorescence in situ hybridization revealed MGEA6-related sequences on different human chromosomes. Here we carry out database analysis to investigate the complexity of the MGEA6-related sequences and demonstrate the existence of a multigene family. We localized the active gene (spanning over 83 kb) to chromosome 14q and elucidated its exon/intron structure. We identified and characterized 9 processed pseudogenes on 9 different chromosomes including chromosomes 2, 3, 6, 7, 9, 10, 12, 13, and 18. We performed phylogenetic analysis and concluded that the MGEA6 pseudogenes may result from more than one retrotransposition event; we calculated divergence times of the pseudogenes to be between 21.5 and 28.9 million years ago.

引用

页码：43 / 48

页数：6

共 23 条

[1] SEQUENCE REQUIREMENTS FOR SPLICING OF HIGHER EUKARYOTIC NUCLEAR PRE-MESSENGER-RNA [J].

AEBI, M ;

HORNIG, H ;

PADGETT, RA ;

REISER, J ;

WEISSMANN, C .

CELL, 1986, 47 (04) :555-565

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3] Tumor antigens [J].

Boon, T ;

Old, LJ .

CURRENT OPINION IN IMMUNOLOGY, 1997, 9 (05) :681-683

[4]

Cotton RGH, 2000, HUM MUTAT, V15, P16, DOI 10.1002/(SICI)1098-1004(200001)15:1<16::AID-HUMU6>3.0.CO

[5]

2-S

[6] Oncogenic proteins as tumor antigens [J].

Disis, ML ;

Cheever, MA .

CURRENT OPINION IN IMMUNOLOGY, 1996, 8 (05) :637-642

[7]

GUTTLER F, 1986, J INHERIT METAB DIS, V1, P58

[8]

Hardison R, 1998, J EXP BIOL, V201, P1099

[9] cDNA cloning and chromosomal mapping of a predicted coiled-coil proline-rich protein immunogenic in meningioma patients [J].

Heckel, D ;

Brass, N ;

Fischer, U ;

Blin, N ;

Steudel, L ;

Tureci, O ;

Fackler, O ;

Zang, KD ;

Meese, E .

HUMAN MOLECULAR GENETICS, 1997, 6 (12) :2031-2041

[10] Structural interpretation of mutations in phenylalanine hydroxylase protein aids in identifying genotype-phenotype correlations in phenylketonuria [J].

Jennings, IG ;

Cotton, RGH ;

Kobe, B .

EUROPEAN JOURNAL OF HUMAN GENETICS, 2000, 8 (09) :683-696

← 1 2 3 →