Fluorescence characterization of IHSS humic substances: Total luminescence spectra with absorbance correction

被引：342

作者：

Mobed, JJ ^{[1
]}

Hemmingsen, SL ^{[1
]}

Autry, JL ^{[1
]}

McGown, LB ^{[1
]}

机构：

[1] DUKE UNIV, DEPT CHEM, PAUL M GROSS CHEM LAB, DURHAM, NC 27708 USA

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 1996年 / 30卷 / 10期

关键词：

D O I：

10.1021/es960132l

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Total luminescence spectroscopy was applied to the fluorescence characterization of humic substances obtained from the International Humic Substances Society (IHSS). Results show that total luminescence spectra, represented as excitation-emission matrices (EEMs), may be used to discriminate between soil-derived and aquatic-derived IHSS humic substances and between humic and fulvic acids derived from the same source (soil or aquatic). Ionic strength in the range of 0-1 M KCl and humic substance concentration in the range of 5-100 mg/L had little effect on the fluorescence spectral characteristics of the humic substances, while pH had significant effects as expected. Absorbance correction was shown to be essential for accurate representation and comparison of the EEMs of the humic substances at high concentrations.

引用

页码：3061 / 3065

页数：5

共 16 条

[11] SPECTROSCOPIC AND COMPOSITIONAL COMPARATIVE CHARACTERIZATION OF IHSS REFERENCE AND STANDARD FULVIC AND HUMIC ACIDS OF VARIOUS ORIGIN [J].

SENESI, N ;

MIANO, TM ;

PROVENZANO, MR ;

BRUNETTI, G .

SCIENCE OF THE TOTAL ENVIRONMENT, 1989, 81-2 :143-156

[12] CHARACTERIZATION, DIFFERENTIATION, AND CLASSIFICATION OF HUMIC SUBSTANCES BY FLUORESCENCE SPECTROSCOPY [J].

SENESI, N ;

MIANO, TM ;

PROVENZANO, MR ;

BRUNETTI, G .

SOIL SCIENCE, 1991, 152 (04) :259-271

[13] MOLECULAR AND QUANTITATIVE ASPECTS OF THE CHEMISTRY OF FULVIC-ACID AND ITS INTERACTIONS WITH METAL-IONS AND ORGANIC-CHEMICALS .2. THE FLUORESCENCE SPECTROSCOPY APPROACH [J].

SENESI, N .

ANALYTICA CHIMICA ACTA, 1990, 232 (01) :77-106

[14]

Senesi N., 1993, ORGANIC SUBSTANCES S, P74

[15]

STEVENSON FJ, 1994, HUMUS CHEM GENESIS C, P31

[16] PRIMARY AND SECONDARY INNER FILTERING - EFFECT OF K2CR2O7 ON FLUORESCENCE EMISSION INTENSITIES OF QUININE SULFATE [J].

TUCKER, SA ;

AMSZI, VL ;

ACREE, WE .

JOURNAL OF CHEMICAL EDUCATION, 1992, 69 (01) :A8-&

← 1 2 →