The role of interleukin-22 in hepatitis C virus infection

被引:76
作者
Dambacher, Julia [1 ]
Beigel, Florian [1 ]
Zitzmann, Kathrin [1 ]
Heeg, Malte H. J. [1 ]
Goeke, Burkhard [1 ]
Diepolder, Helmut M. [1 ]
Auernhammer, Christoph J. [1 ]
Brand, Stephan [1 ]
机构
[1] Univ Munich, Univ Hosp Munich Grosshadern, Dept Med 2, D-81377 Munich, Germany
关键词
IL-22; viral hepatitis; hepatitis B (HBV); hepatitis C (HCV); interferon-alpha;
D O I
10.1016/j.cyto.2007.11.016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, we analyzed if IL-22 displays, similar to other IL-10 like cytokines such as IL-28A, antiviral properties in hepatic cells. Using RT-PCR and immunoblotting, we demonstrated that hepatic cell lines and primary hepatocytes express the functional IL-22 receptor complex consisting of IL-22R1 and IL-10R2. Hepatic IL-22 mRNA expression as measured by quantitative PCR was up-regulated in autoimmune and viral hepatitis compared to cholestatic liver diseases, while IL-22 serum levels did not differ significantly between patients with viral hepatitis and normal controls. IL-22 did not significantly change the expression levels of 1FN-alpha/-beta and of the antiviral proteins MxA and 2',5'-OAS. Consequently, it had in comparison to IFN-alpha no relevant antiviral activity in in vitro models of HCV replication and infection. Taken together, hepatic IL-22 expression is up-regulated in viral hepatitis but IL-22 does not directly regulate antiviral proteins and has, in contrast to IFN-alpha, no effect on HCV replication. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:209 / 216
页数:8
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