The role of interleukin-22 in hepatitis C virus infection

被引：76

作者：

Dambacher, Julia ^{[1
]}

Beigel, Florian ^{[1
]}

Zitzmann, Kathrin ^{[1
]}

Heeg, Malte H. J. ^{[1
]}

Goeke, Burkhard ^{[1
]}

Diepolder, Helmut M. ^{[1
]}

Auernhammer, Christoph J. ^{[1
]}

Brand, Stephan ^{[1
]}

机构：

[1] Univ Munich, Univ Hosp Munich Grosshadern, Dept Med 2, D-81377 Munich, Germany

来源：

CYTOKINE | 2008年 / 41卷 / 03期

关键词：

IL-22; viral hepatitis; hepatitis B (HBV); hepatitis C (HCV); interferon-alpha;

D O I：

10.1016/j.cyto.2007.11.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we analyzed if IL-22 displays, similar to other IL-10 like cytokines such as IL-28A, antiviral properties in hepatic cells. Using RT-PCR and immunoblotting, we demonstrated that hepatic cell lines and primary hepatocytes express the functional IL-22 receptor complex consisting of IL-22R1 and IL-10R2. Hepatic IL-22 mRNA expression as measured by quantitative PCR was up-regulated in autoimmune and viral hepatitis compared to cholestatic liver diseases, while IL-22 serum levels did not differ significantly between patients with viral hepatitis and normal controls. IL-22 did not significantly change the expression levels of 1FN-alpha/-beta and of the antiviral proteins MxA and 2',5'-OAS. Consequently, it had in comparison to IFN-alpha no relevant antiviral activity in in vitro models of HCV replication and infection. Taken together, hepatic IL-22 expression is up-regulated in viral hepatitis but IL-22 does not directly regulate antiviral proteins and has, in contrast to IFN-alpha, no effect on HCV replication. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：209 / 216

页数：8

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