In vitro analysis of protein-operator interactions of the NikR and fur metal-responsive regulators of coregulated genes in Helicobacter pylori

被引:83
作者
Delany, I
Ieva, R
Soragni, A
Hilleringmann, M
Rappuoli, R
Scarlato, V
机构
[1] Chiron Vaccines, Mol Immunol Unit, I-53100 Siena, Italy
[2] Chiron Vaccines, Cellular Microbiol & Bioinformat Unit, I-53100 Siena, Italy
[3] Univ Bologna, Dept Biol, I-40126 Bologna, Italy
关键词
D O I
10.1128/JB.187.22.7703-7715.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two important metal-responsive regulators, NikR and Fur, are involved in nickel and iron homeostasis and controlling gene expression in Helicobacter pylori. To date, they have been implicated in the regulation of sets of overlapping genes. We have attempted here dissection of the molecular mechanisms involved in transcriptional regulation of the NikR and Fur proteins, and we investigated protein-promoter interactions of the regulators with known target genes. We show that H. pylori NikR is a tetrameric protein and, through DNase I footprinting analysis, we have identified operators for NikR to which it binds with different affinities in a metal-responsive way. Mapping of the NikR binding site upstream of the urease promoter established a direct role for NikR as a positive regulator of transcription and, through scanning mutagenesis of this binding site, we have determined two subsites that are important for the binding of the protein to its target sequence. Furthermore, by alignment of the operators for NikR, we have shown that the H. pylori protein recognizes a sequence that is distinct from its well-studied orthologue in Escherichia coli. Moreover, we show that NikR and Fur can bind independently at distinct operators and also compete for overlapping operators in some coregulated gene promoters, adding another dimension to the previous suggested link between iron and nickel regulation. Finally, the importance of an interconnection between metal-responsive gene networks for homeostasis is discussed.
引用
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页码:7703 / 7715
页数:13
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