Applications of CRISPR technologies in research and beyond

被引：636

作者：

Barrangou, Rodolphe ^{[1
]}

Doudna, Jennifer A. ^{[2
,3
,4
]}

机构：

[1] North Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA

[2] Univ Calif Berkeley, Howard Hughes Med Inst, Innovat Genom Initiat, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[4] Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

来源：

NATURE BIOTECHNOLOGY | 2016年 / 34卷 / 09期

关键词：

HEPATITIS-B-VIRUS; PLURIPOTENT STEM-CELLS; RNA-GUIDED ENDONUCLEASE; SEQUENCE-SPECIFIC ANTIMICROBIALS; DUCHENNE MUSCULAR-DYSTROPHY; STAPHYLOCOCCUS-AUREUS CAS9; TARGET DNA RECOGNITION; IN-VIVO; HIV-1; INFECTION; STREPTOCOCCUS-THERMOPHILUS;

D O I：

10.1038/nbt.3659

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Programmable DNA cleavage using CRISPR-Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms. In the research arena, versatile CRISPR-enabled genome editing has been used in various ways, such as controlling transcription, modifying epigenomes, conducting genome-wide screens and imaging chromosomes. CRISPR systems are already being used to alleviate genetic disorders in animals and are likely to be employed soon in the clinic to treat human diseases of the eye and blood. Two clinical trials using CRISPR-Cas9 for targeted cancer therapies have been approved in China and the United States. Beyond biomedical applications, these tools are now being used to expedite crop and livestock breeding, engineer new antimicrobials and control disease-carrying insects with gene drives.

引用

页码：933 / 941

页数：9

共 204 条

[1] Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System [J].

Ali, Zahir ;

Abul-faraj, Aala ;

Li, Lixin ;

Ghosh, Neha ;

Piatek, Marek ;

Mahjoub, Ali ;

Aouida, Mustapha ;

Piatek, Agnieszka ;

Baltes, Nicholas J. ;

Voytas, Daniel F. ;

Dinesh-Kumar, Savithramma ;

Mahfouz, Magdy M. .

MOLECULAR PLANT, 2015, 8 (08) :1288-1291

[2] In Vitro Reconstitution and Crystallization of Cas9 Endonuclease Bound to a Guide RNA and a DNA Target [J].

Anders, Carolin ;

Niewoehner, Ole ;

Jinek, Martin .

STRUCTURES OF LARGE RNA MOLECULES AND THEIR COMPLEXES, 2015, 558 :515-537

[3] In Vitro Enzymology of Cas9 [J].

Anders, Carolin ;

Jinek, Martin .

USE OF CRISPR/CAS9, ZFNS, AND TALENS IN GENERATING SITE-SPECIFIC GENOME ALTERATIONS, 2014, 546 :1-20

[4] Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease [J].

Anders, Carolin ;

Niewoehner, Ole ;

Duerst, Alessia ;

Jinek, Martin .

NATURE, 2014, 513 (7519) :569-+

[5] Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity [J].

Anderson, Emily M. ;

Haupt, Amanda ;

Schiel, John A. ;

Chou, Eldon ;

Machado, Hidevaldo B. ;

Strezoska, Zaklina ;

Lenger, Steve ;

McClelland, Shawn ;

Birmingham, Amanda ;

Vermeulen, Annaleen ;

Smith, Anja van Brabant .

JOURNAL OF BIOTECHNOLOGY, 2015, 211 :56-65

[6] In Vivo CRISPR/Cas9 Gene Editing Corrects Retinal Dystrophy in the S334ter-3 Rat Model of Autosomal Dominant Retinitis Pigmentosa [J].

Bakondi, Benjamin ;

Lv, Wenjian ;

Lui, Bin ;

Jones, Melissa K. ;

Tsai, Yuchun ;

Kim, Kevin J. ;

Levy, Rachelle ;

Akhtar, Aslam Abbasi ;

Breunig, Joshua J. ;

Svendseni, Clive N. ;

Wang, Shaomei .

MOLECULAR THERAPY, 2016, 24 (03) :556-563

[7] A prudent path forward for genomic engineering and germline gene modification [J].

Baltimore, David ;

Berg, Paul ;

Botchan, Michael ;

Carroll, Dana ;

Charo, R. Alta ;

Church, George ;

Corn, Jacob E. ;

Daley, George Q. ;

Doudna, Jennifer A. ;

Fenner, Marsha ;

Greely, Henry T. ;

Jinek, Martin ;

Martin, G. Steven ;

Penhoet, Edward ;

Puck, Jennifer ;

Sternberg, Samuel H. ;

Weissman, Jonathan S. ;

Yamamoto, Keith R. .

SCIENCE, 2015, 348 (6230) :36-38

[8] CRISPR provides acquired resistance against viruses in prokaryotes [J].

Barrangou, Rodolphe ;

Fremaux, Christophe ;

Deveau, Helene ;

Richards, Melissa ;

Boyaval, Patrick ;

Moineau, Sylvain ;

Romero, Dennis A. ;

Horvath, Philippe .

SCIENCE, 2007, 315 (5819) :1709-1712

[9] Exploiting CRISPR-Cas immune systems for genome editing in bacteria [J].

Barrangou, Rodolphe ;

van Pijkeren, Jan-Peter .

CURRENT OPINION IN BIOTECHNOLOGY, 2016, 37 :61-68

[10] Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference [J].

Barrangou, Rodolphe ;

Birmingham, Amanda ;

Wiemann, Stefan ;

Beijersbergen, Roderick L. ;

Hornung, Veit ;

Smith, Anja van Brabant .

NUCLEIC ACIDS RESEARCH, 2015, 43 (07) :3407-3419

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