Nanostructured Aptamer-Functionalized Black Phosphorus Sensing Platform for Label-Free Detection of Myoglobin, a Cardiovascular Disease Biomarker

被引：240

作者：

Kumar, Vinod ^{[1
]}

Brent, Jack R. ^{[2
]}

Shorie, Munish ^{[1
]}

Kaur, Harmanjit ^{[1
]}

Chadha, Gaganpreet ^{[1
]}

Thomas, Andrew G. ^{[2
]}

Lewis, Edward A. ^{[2
]}

Rooney, Aidan P. ^{[2
]}

Lan Nguyen ^{[2
]}

Zhong, Xiang Li ^{[2
]}

Burke, M. Grace ^{[2
]}

Haigh, Sarah J. ^{[2
]}

Walton, Alex ^{[3
]}

McNaughter, Paul D. ^{[3
]}

Tedstone, Aleksander A. ^{[3
]}

Savjani, Nicky ^{[3
]}

Muryn, Christopher A. ^{[3
]}

O'Brien, Paul ^{[2
,3
]}

Ganguli, Ashok K. ^{[1
,4
]}

Lewis, David J. ^{[2
,3
]}

Sabherwal, Priyanka ^{[1
]}

机构：

[1] Habitat Ctr, Inst Nano Sci & Technol, Sect 64, Mohali 160062, Punjab, India

[2] Univ Manchester, Sch Mat, Oxford Rd, Manchester M13 9PL, Lancs, England

[3] Univ Manchester, Sch Chem, Oxford Rd, Manchester M13 9PL, Lancs, England

[4] Indian Inst Technol Delhi, Dept Chem, Hauz Khas, New Delhi 110016, India

来源：

ACS APPLIED MATERIALS & INTERFACES | 2016年 / 8卷 / 35期

基金：

英国工程与自然科学研究理事会;

关键词：

sensing heart disease; myoglobin; aptamer; 2-D materials; black phosphorus; ACUTE MYOCARDIAL-INFARCTION; GRAPHENE; EXFOLIATION; MOS2; NANOCOMPOSITES; SPECTROSCOPY; TRANSISTORS; DIAGNOSIS; LEUKEMIA;

D O I：

10.1021/acsami.6b06488

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

We report the electrochemical detection of the redox active cardiac biomarker myoglobin (Mb) using aptamer-functionalized black phosphorus nanostructured electrodes by measuring direct electron transfer. The as synthesized few-layer black phosphorus nanosheets have been functionalized with poly-L-lysine (PLL) to facilitate binding with generated anti-Mb DNA aptamers on nanostructured electrodes. This aptasensor platform has a record-low detection limit (similar to 0.524 pg mL(-1)) and sensitivity (36 mu A pg(-1) mL cm(-2)) toward Mb with a dynamic response range from 1 pg mL(-1) to 16 mu g mL(-1) for Mb in serum samples. This strategy opens up avenues to bedside technologies for multiplexed diagnosis of cardiovascular diseases in complex human samples.

引用

页码：22860 / 22868

页数：9

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