Analysis of membrane-bound complement regulatory proteins in prostate cancer

被引:34
作者
Loberg, RD
Wojno, KJ
Day, LL
Pienta, KJ
机构
[1] Univ Michigan Hlth Syst, Michigan Urol Ctr, Dept Urol, Ann Arbor, MI 48109 USA
[2] Univ Michigan Hlth Syst, Dept Internal Med, Ann Arbor, MI 48109 USA
关键词
D O I
10.1016/j.urology.2005.06.094
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Objectives. Membrane-bound complement regulatory proteins (mCRPs) are ubiquitously expressed throughout the cells of the hematopoietic system and protect host cells from complement-mediated lysis. The expression and functionality of mCRPs have been demonstrated in several types of cancers; however, no clear correlation has been appreciated between tumor stage and mCRP expression. Methods. We investigated the expression pattern of CD46, CD55, CD59, and CD97 (a receptor for CD55) in prostate cancer by tissue microarray analysis using high-density tissue microarrays spotted with tissue cores from biopsy and autopsy specimens. Real-time polymerase chain reaction was used to confirm the tissue microarray data by quantifying mRNA expression in clinically identified tissue specimens. Results. CD55 and CD97 demonstrated increased expression in prostatic intraepithelial neoplasia, localized prostate cancer, and metastatic prostate cancer specimens compared with normal tissue specimens. No appreciable difference was seen in CD46 or CD59 expression. These data were confirmed by real-time polymerase chain reaction of mRNA expression levels in grossly identified specimens. Conclusions. These data suggest that mCRPs are differentially expressed in prostate cancer, with CD55 the predominant mCRP upregulated in malignant prostate epithelial cells. Additionally, CD97 expression correlated with the malignant phenotype and may contribute to the tumorigenic and metastatic potential of prostate cancer.
引用
收藏
页码:1321 / 1326
页数:6
相关论文
共 18 条
[11]   Hypocomplementemia in multiple myeloma [J].
Lugassy, G ;
Platok, I ;
Schlesinger, M .
LEUKEMIA & LYMPHOMA, 1999, 33 (3-4) :365-370
[12]   Randomized double-blind phase II survival study comparing immunization with the anti idiotypic monoclonal antibody 105AD7 against placebo in advanced colorectal cancer [J].
Maxwell-Armstrong, CA ;
Durrant, LG ;
Buckley, TJD ;
Scholefield, JH ;
Robins, RA ;
Fielding, K ;
Monson, JRT ;
Guillou, P ;
Calvert, H ;
Carmichael, J ;
Hardcastle, JD .
BRITISH JOURNAL OF CANCER, 2001, 84 (11) :1443-1446
[13]   The role of CD55 in protecting the tumour environment from complement attack [J].
Morgan, J ;
Spendlove, I ;
Durrant, LG .
TISSUE ANTIGENS, 2002, 60 (03) :213-223
[14]  
Niehans GA, 1996, AM J PATHOL, V149, P129
[15]   E-cadherin expression in prostate cancer: A broad survey using high-density tissue microarray technology [J].
Rubin, MA ;
Mucci, NR ;
Figurski, J ;
Fecko, A ;
Pienta, KJ ;
Day, ML .
HUMAN PATHOLOGY, 2001, 32 (07) :690-697
[16]   Analysis of the level of mRNA expression of the membrane regulators of complement, Cd59, Cd55 and Cd46, in breast, cancer [J].
Rushmere, NK ;
Knowlden, JM ;
Gee, JMW ;
Harper, ME ;
Robertson, JF ;
Morgan, BP ;
Nicholson, RI .
INTERNATIONAL JOURNAL OF CANCER, 2004, 108 (06) :930-936
[17]   Increased CD59 protein expression predicts a PSA relapse in patients after radical prostatectomy [J].
Xu, CL ;
Jung, M ;
Burkhardt, M ;
Stephan, C ;
Schnorr, D ;
Loening, S ;
Jung, K ;
Dietel, M ;
Kristiansen, G .
PROSTATE, 2005, 62 (03) :224-232
[18]  
ZURLO JJ, 1989, AM J MED, V87, P411