Effect of long-term treatment with aromatase inhibitor on testicular function of adult male bonnet monkeys (M-radiata)

被引:72
作者
Shetty, G
Krishnamurthy, H
Krishnamurthy, HN
Bhatnagar, AS
Moudgal, NR
机构
[1] Indian Inst Sci, Dept Mol Reprod Dev & Genet, Bangalore 560012, Karnataka, India
[2] Ciba Geigy Ltd, Basel, Switzerland
关键词
estrogen; primate; spermatogenesis; testicular function;
D O I
10.1016/S0039-128X(98)00042-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The role/need for estrogen in regulating testicular function of adult male bonnet monkeys (M. radiata) has been investigated by dosing orally a group of five normal males 2.5 mgs of CGP 47645, a long-acting nonsteroidal aromatase inhibitor (AI), once every 5 days for over 150 days. Such treatment resulted in a 10-fold increment in nocturnal serum testosterone (T) levels, which were sustained for 85 days of treatment, and a twofold increment in basal serum T levels was present throughout the 150 days of treatment. Analysis of ejaculated semen showed a marked reduction (similar to 90%) in sperm counts in four out of five monkeys between Days 55-85 of treatment. During this period, the motility scare also was markedly reduced from a normal score of 3-5 to 0-2. Flow cytometric analysis of testicular germ cells obtained from biopsy tissue taken on Days 63 and 120 indicated a marked reduction only in elongating/elongated spermatid population (compared to Day 0 values), suggesting inhibition in spermiogenic process. Epididymal sperm maturation also seemed effected as sperm chromatin, on flow cytometric analysis for decondensability following exposure to 5 mM dithiotreitol, showed to be in a hypercondensed state. This study thus indicates that estrogen has an important role in providing normal testicular and sperm function in the primate. (C) 1996 by Elsevier Science Inc.
引用
收藏
页码:414 / 420
页数:7
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