Disruption of a gene encoding phosphatidic acid phosphatase causes abnormal phenotypes in cell growth and abnormal cytokinesis in Saccharomyces cerevisiae

被引：8

作者：

Katagiri, T

Shinozaki, K

机构：

[1] Inst Phys & Chem Res, Tsukuba Life Sci Ctr, RIKEN, Plant Mol Biol Lab, Tsukuba, Ibaraki 3050074, Japan

[2] Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3050006, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 01期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

D O I：

10.1006/bbrc.1998.8914

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphatidic acid phosphatase (PAP) is an enzyme involved in lipid metabolism. Diacylglycerol (DG) and phosphatidic acid (PA) are a substrate and a product of PAP, respectively, and function as second messengers in several signal transduction pathways in animals. To investigate the function of PAP in Saccharomyces cerevisiae, we analyzed changes in cellular phenotypes of a mutant that has a disrupted PAP gene. Two putative genes for PAP (ScPAP1 and ScPAP2) are in the S. cerevisiae genome. We generated a ScPAP1-null mutant and observed its cellular phenotypic changes. The ScPAP1-null mutant cells aggregated in liquid culture, and microscopical analyses showed that these mutant cells have an abnormal cell shape and abnormal cytokinesis during cell division. The ScPAP1 is possibly involved in cell growth and cytokinesis in S. cerevisiae. Yeast phosphatidic acid phosphatase is possibly involved in cell growth and cytokinesis, (C) 1998 Academic Press.

引用

页码：87 / 92

页数：6

共 22 条

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