CLONING OF THE CANDIDA-GLABRATA TRP1 AND HIS3 GENES, AND CONSTRUCTION OF THEIR DISRUPTANT STRAINS BY SEQUENTIAL INTEGRATIVE TRANSFORMATION

被引：134

作者：

KITADA, K

YAMAGUCHI, E

ARISAWA, M

机构：

[1] Department of Mycology, Nippon Roche Research Center, Kamakura, Kanagawa

来源：

GENE | 1995年 / 165卷 / 02期

关键词：

PATHOGENIC FUNGUS; PLASMID VECTOR; HOST STRAIN; YEAST; SELECTION MARKER;

D O I：

10.1016/0378-1119(95)00552-H

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Candida glabrata (Cg) TRP1 and HIS3 genes have been isolated by complementation of the Saccharomyces cerevisiae (Sc) trp1 and his3 mutants, respectively. Cg TRP1 encodes a polypeptide of 217 amino acids (aa), whose aa sequence is 58% identical to that of Sc TRP1. Cg HIS3 encodes a polypeptide of 210 aa, whose aa sequence is 73% identical to that of the Sc HIS3. Both Cg TRP1 and HIS3 were disrupted by sequential integrative transformation where the Sc URA3 was used as a selection marker for transformation. The resulting auxotrophic strain of his3(-) and trp1(-) was used to examine the ability of the Sc genes to complement the Cg mutations; Sc HIS3 and TRP1 complemented the Cg his3(-) and trp1(-) mutations, respectively.

引用

页码：203 / 206

页数：4

共 19 条

[1] TORULOPSIS-GLABRATA INFECTIONS IN PATIENTS WITH CANCER - INCREASING INCIDENCE AND RELATIONSHIP TO COLONIZATION [J].