Effect of bovine lactoferrin on extracellular matrix calcification by human osteoblast-like cells

被引：36

作者：

Takayama, Yoshiharu ^{[1
]}

Mizumachi, Koko ^{[1
]}

机构：

[1] Natl Inst Livestock & Grassland Sci, Funct Biomol Res Team, Tsukuba, Ibaraki 3050901, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 01期

关键词：

alkaline phosphatase; calcium deposition; osteocalcin; osteogenesis; MG63; cells;

D O I：

10.1271/bbb.70465

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Osteoblast-mediated calcium deposition to the extracellular matrix (ECM) is a critical step in bone tissue generation. Bovine lactoferrin enhanced the calcium deposition by MG63 human osteoblast-like cells cultured on collagen-coated plates. Lactoferrin also promoted the alkaline phosphatase activity and osteocalcin production during the calcification process, whereas it had little effect on the growth of the cells on the collagen-coated plates.

引用

页码：226 / 230

页数：5

共 16 条

[1]

BONEWALD LF, 1992, J BIOL CHEM, V267, P8943

[2] The physiology of lactoferrin [J].

Brock, JH .

BIOCHEMISTRY AND CELL BIOLOGY, 2002, 80 (01) :1-6

[3] Bio-adhesive surfaces to promote osteoblast differentiation and bone formation [J].

García, AJ ;

Reyes, CD .

JOURNAL OF DENTAL RESEARCH, 2005, 84 (05) :407-413

[4]

Lönnerdal B, 2003, AM J CLIN NUTR, V77, p1537S

[5] Osteoblasts: novel roles in orchestration of skeletal architecture [J].

Mackie, EJ .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2003, 35 (09) :1301-1305

[6]

Meyer U, 2005, Eur Cell Mater, V9, P39

[7] The soybean isoflavone genistein induces differentiation of MG63 human osteosarcoma osteoblasts [J].

Morris, C ;

Thorpe, J ;

Ambrosio, L ;

Santin, M .

JOURNAL OF NUTRITION, 2006, 136 (05) :1166-1170

[8] Lactoferrin - A Novel Bone Growth Factor [J].

Naot, Dorit ;

Grey, Andrew ;

Reid, Ian R. ;

Cornish, Jillian .

CLINICAL MEDICINE & RESEARCH, 2005, 3 (02) :93-101

[9]

Narisawa S, 1997, DEV DYNAM, V208, P432, DOI 10.1002/(SICI)1097-0177(199703)208:3<432::AID-AJA13>3.0.CO

[10]

2-1

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