Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition

被引：1260

作者：

Griffiths, RI

Whiteley, AS

O'Donnell, AG

Bailey, MJ

机构：

[1] CEH Oxford, Inst Virol & Environm Microbiol, Mol Microbial Ecol Lab, Oxford OX1 3SR, England

[2] Univ Newcastle Upon Tyne, Dept Agr & Environm Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 12期

关键词：

D O I：

10.1128/AEM.66.12.5488-5491.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction, Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.

引用

页码：5488 / 5491

页数：4

共 15 条

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