Important role of EP4, a subtype of prostaglandin (PG) E receptor, in osteoclast-like cell formation from mouse bone marrow cells induced by PGE2

被引：39

作者：

Ono, K ^{[1
]}

Akatsu, T ^{[1
]}

Murakami, T ^{[1
]}

Nishikawa, M ^{[1
]}

Yamamoto, M ^{[1
]}

Kugai, N ^{[1
]}

Motoyoshi, K ^{[1
]}

Nagata, N ^{[1
]}

机构：

[1] Natl Def Med Coll, Dept Internal Med 3, Tokorozawa, Saitama 359, Japan

来源：

JOURNAL OF ENDOCRINOLOGY | 1998年 / 158卷 / 03期

关键词：

D O I：

10.1677/joe.0.158R001

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Of various PGs, PGE(1) and PGE(2) are shown to be the most potent stimulators of osteoclastogenesis in vitro. PGE receptors have been classified into four subtypes, EP1-EP4. Little is known about PGE receptors functioning in bone cells. In this study, using mouse marrow culture, we investigated which PGE receptors are important in osteoclast-like cell (OCL) formation induced by PGE. 11-deoxy-PGE(1) (EP2, EP3 and EP4 agonist) stimulated OCL formation potently. Butaprost (EP2 agonist) stimulated it slightly, while sulprostone (EP1 and EP3 agonist) and ONO-AP-324-01 (EP3 agonist) did not. AH23848B (EP4 antagonist) inhibited PGE(2)-induced OCL formation in a dose-dependent manner. The expression of EP4 mRNA in mouse bone marrow was confirmed by RT-PCR. The results indicate an important role of EP4 in PGE(2) induced OCL formation in marrow cultures and suggest therapeutic potential of EP4 antagonists in some clinical conditions with accelerated bone resorption.

引用

页码：R1 / R5

页数：5

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