Protein pyrophosphorylation by inositol pyrophosphates is a posttranslational event

被引：171

作者：

Bhandari, Rashna

Saiardi, Adolfo

Ahmadibeni, Yousef

Snowman, Adele M.

Resnick, Adam C.

Kristiansen, Troels Z.

Molina, Henrik

Pandey, Akhilesh

Werner, J. Kent, Jr.

Juluri, Krishna R.

Xu, Yong

Prestwich, Glenn D.

Parang, Keykavous

Snyder, Solomon H.

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA

[4] Johns Hopkins Univ, Sch Med, Nathans Inst Genet Med, Baltimore, MD 21205 USA

[5] Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA

[6] UCL, Dept Biochem & Mol Biol, Mol Cell Biol Lab, London WC1E 6BT, England

[7] UCL, MRC, Cell Biol Unit, London WC1E 6BT, England

[8] Univ Rhode Isl, Coll Pharm, Dept Biomed & Pharmaceut Sci, Kingston, RI 02881 USA

[9] Univ Utah, Dept Med Chem, Salt Lake City, UT 84108 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 39期

基金：

英国医学研究理事会;

关键词：

inositol polyphosphate; protein phosphorylation;

D O I：

10.1073/pnas.0707338104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In a previous study, we showed that the inositol pyrophosphate diphosphoinositol pentakisphosphate (IP7) physiologically phosphorylates mammalian and yeast proteins. We now report that this phosphate transfer reflects pyrophosphorylation. Thus, proteins must be prephosphorylated by ATIP to prime them for IP7 phosphorylation. IP7 phosphorylates synthetic phosphopeptides but not if their phosphates have been masked by methylation or pyrophosphorylation. Moreover, IP7 phosphorylated peptides are more acid-labile and more resistant to phosphatases than ATIP phosphorylated peptides, indicating a different type of phosphate bond. Pyrophosphorylation may represent a novel mode of signaling to proteins.

引用

页码：15305 / 15310

页数：6

共 27 条

[1] Inositol pyrophosphates: metabolism and signaling [J].