Conjugated linoleic acid determination in human milk by fast-gas chromatography

被引：43

作者：

Molto-Puigmarti, Carolina ^{[1
]}

Castellote, Anna Isabel ^{[1
]}

Lopez-Sabater, M. Carmen ^{[1
]}

机构：

[1] Univ Barcelona, Fac Pharm, Dept Nutr & Food Sci, Food Technol Refernce Ctr, E-08028 Barcelona, Spain

来源：

ANALYTICA CHIMICA ACTA | 2007年 / 602卷 / 01期

关键词：

human milk; rat milk; fast-gas chromatography; conjugated linoleic acid; rumenic acid; fatty acids;

D O I：

10.1016/j.aca.2007.09.011

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An efficient direct method for measuring c9,t11- and t10,c12-conjugated linoleic acid (CIA) isomer content in human and rat milk was developed and validated using an RTX-2330 capillary column (40 m x 0.18 mm x 0.1 mu m). In comparison with the commonly used 100 m x 0.25 mm x 0.20 mu m columns, this new type of fast column allowed the separation of FAMEs with the same resolution but in much less time. An additional advantage for biological samples was that only a small volume of sample was needed. Two different procedures were tested in order to select the best methylation of CIA isomers, and the alkali plus acid-catalyzed procedure was selected. The precision results showed relative standard deviations (R.S.D.) of repeatability and reproducibility ranging between 0.10 and 8.71%. The application of this method to human and rat milk samples showed that it was a rapid, simple and reliable method for the analysis of biological samples. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：122 / 130

页数：9

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