Micromachined heated chemical reactor for pre-column derivatisation

被引：26

作者：

Eijkel, JCT

Prak, A

Cowen, S

Craston, DH

Manz, A ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Zeneca SmithKline Beecham Ctr Analyt Sci, London SW7 2AY, England

[2] Lab Govt Chemist, Teddington TW11 0LY, Middx, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 815卷 / 02期

关键词：

microreactor; derivatization; LC; chip technology; micro-total analysis system; amino acids; 4-fluoro-7-nitrobenzofuran; sodium azide;

D O I：

10.1016/S0021-9673(98)00459-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A micromachined heated chemical reactor is presented. The microreactor consists of a sandwich of glass and silicon chips and contains a reactor channel of 50 mu l volume. On-chip heating resistors supply a power of 2 W, resulting in a heating rate of 2 degrees C/s. On-chip measuring resistors enable feed-back control of the temperature. The reactor volume makes the device suitable for derivatisation purposes in HPLC, As an example the pre-column derivatisation of amino acids with NBD-F is demonstrated, requiring 2 min of heating at 60 degrees C, followed by HPLC separation and fluorescence detection. (C) 1998 Elsevier Science BN. All rights reserved.

引用

页码：265 / 271

页数：7

共 16 条

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