Micromachined heated chemical reactor for pre-column derivatisation

被引:26
作者
Eijkel, JCT
Prak, A
Cowen, S
Craston, DH
Manz, A [1 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Zeneca SmithKline Beecham Ctr Analyt Sci, London SW7 2AY, England
[2] Lab Govt Chemist, Teddington TW11 0LY, Middx, England
关键词
microreactor; derivatization; LC; chip technology; micro-total analysis system; amino acids; 4-fluoro-7-nitrobenzofuran; sodium azide;
D O I
10.1016/S0021-9673(98)00459-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A micromachined heated chemical reactor is presented. The microreactor consists of a sandwich of glass and silicon chips and contains a reactor channel of 50 mu l volume. On-chip heating resistors supply a power of 2 W, resulting in a heating rate of 2 degrees C/s. On-chip measuring resistors enable feed-back control of the temperature. The reactor volume makes the device suitable for derivatisation purposes in HPLC, As an example the pre-column derivatisation of amino acids with NBD-F is demonstrated, requiring 2 min of heating at 60 degrees C, followed by HPLC separation and fluorescence detection. (C) 1998 Elsevier Science BN. All rights reserved.
引用
收藏
页码:265 / 271
页数:7
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