Inhibition of Vibrio cholerae biofilm by AiiA enzyme produced from Bacillus spp.

被引：46

作者：

Augustine, Nimmy ^{[1
]}

Kumar, Praveen ^{[1
]}

Thomas, Sabu ^{[1
]}

机构：

[1] Rajiv Gandhi Ctr Biotechnol, Dept Mol Microbiol, Cholera & Environm Microbiol Lab, Trivandrum 695014, Kerala, India

来源：

ARCHIVES OF MICROBIOLOGY | 2010年 / 192卷 / 12期

关键词：

AiiA enzyme; Biofilm; Quorum sensing; V; cholerae; QUORUM; THURINGIENSIS; LACTONASE; CEREUS; GENE; PCR; DIFFERENTIATION; BACTERIA; ENHANCE;

D O I：

10.1007/s00203-010-0633-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Vibrio cholerae is the causative agent of water-borne diarrheal disease, cholera. The formation of biofilm favors survival and persistence of V. cholerae in the aquatic environment and also inside the host. AHL lactonase (AiiA), a metallo-beta-lactamase produced by Bacillus spp., blocks quorum sensing in Gram-negative bacteria by hydrolyzing N-acyl-homoserine lactones (AHLs). In the present investigation, AiiA-mediated inhibition of V. cholerae biofilm was studied. Two novel alleles of aiiA-encoding genes from Bacillus spp. were expressed in E. coli, and the results demonstrated that AiiA enzyme is a potent inhibitor of V. cholerae biofilm.

引用

收藏

页码：1019 / 1022

页数：4

相关论文

共 19 条

[1] The Ti plasmid of Agrobactetium tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-acyl homoserine lactonase activity [J].

Carlier, A ;

Uroz, S ;

Smadja, B ;

Fray, R ;

Latour, X ;

Dessaux, Y ;

Faure, D .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (08) :4989-4993

[2] PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells [J].

Chang, YH ;

Shangkuan, YH ;

Lin, HC ;

Liu, HW .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (08) :4502-4510

[3] Disruption of quorum sensing in Vibrio harveyi by the AiiA protein of Bacillus thuringiensis [J].

Fangfang Bai ;

Yin Han ;

Jixiang Chen ;

Xiao-Hua Zhang .

AQUACULTURE, 2008, 274 (01) :36-40

[4] Transition state analogs of 5′-methylthioadenosine nucleosidase disrupt quorum sensing [J].

Gutierrez, Jemy A. ;

Crowder, Tamara ;

Rinaldo-Matthis, Agnes ;

Ho, Meng-Chiao ;

Almo, Steven C. ;

Schramm, Vern L. .

NATURE CHEMICAL BIOLOGY, 2009, 5 (04) :251-257

[5] Quorum sensing controls biofilm formation in Vibrio cholerae [J].

Hammer, BK ;

Bassler, BL .

MOLECULAR MICROBIOLOGY, 2003, 50 (01) :101-114

[6] Quorum-regulated biofilms enhance the development of conditionally viable, environmental Vibrio cholerae [J].

Kamruzzaman, M. ;

Udden, S. M. Nashir ;

Cameron, D. Ewen ;

Calderwood, Stephen B. ;

Nair, G. Balakrish ;

Mekalanos, John J. ;

Faruque, Shah M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (04) :1588-1593

[7] Environmental determinants of Vibrio cholerae biofilm development [J].

Kierek, K ;

Watnick, PI .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (09) :5079-5088

[8] Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis [J].

Lee, SJ ;

Park, SY ;

Lee, JJ ;

Yum, DY ;

Koo, BT ;

Lee, JK .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (08) :3919-3924

[9] Bacillus cereus, Bacillus thuringiensis and Bacillus mycoides differentiation using a PCR-RE technique [J].

Manzano, M ;

Cocolin, L ;

Cantoni, C ;

Comi, G .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 81 (03) :249-254

[10] Expression and characterization of aiiA gene from Bacillus subtilis BS-1 [J].

Pana, Jieru ;

Huang, Tianeei ;

Yao, Fan ;

Huang, Zhipeng ;

Powell, Charles A. ;

Qiu, Sixin ;

Guan, Xiong .

MICROBIOLOGICAL RESEARCH, 2008, 163 (06) :711-716