Structural determination of a 5-O-methyl-deaminated neuraminic acid (Kdn)-containing polysaccharide isolated from Sinorhizobium fredii

被引:37
作者
Gil-Serrano, AM [1 ]
Rodríguez-Carvajal, MA
Tejero-Mateo, P
Espartero, JL
Thomas-Oates, J
Ruiz-Sainz, JE
Buendía-Clavería, AM
机构
[1] Univ Seville, Fac Quim, Dept Quim Organ, E-41071 Seville, Spain
[2] Univ Seville, Fac Farm, Dept Quim Organ & Farmaceut, E-41071 Seville, Spain
[3] Univ Utrecht, Bijvoet Ctr Biomol Res, Dept Mass Spectrometry, NL-3584 CA Utrecht, Netherlands
[4] Univ Seville, Fac Biol, Dept Microbiol, Seville 41071, Spain
关键词
D O I
10.1042/bj3340585
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of a polysaccharide from Sinorhizobium fredii SVQ293, a thiamine auxotrophic mutant of S. fredii HH103, has been determined. This polysaccharide was isolated following the protocol for lipopolysaccharide extraction. On the basis of monosaccharide analysis, methylation analysis, fast atom bombardment MS, collision-induced dissociation tandem MS, one-dimensional H-1 and C-13 NMR and two-dimensional NMR experiments, the structure was shown to consist of the following trisaccharide repeating unit --> 2)-alpha-D-Galp-(1 --> 2)-beta-D-Ribf-(1 --> 9)-alpha-5-O-Me-Kdnp-(2 -->,in which Kdn stands for deaminated neuraminic acid; 25 % of the Kdn residues are not methylated. The structure of this polysaccharide is novel and this is the first report of the presence of Kdn in a rhizobial polysaccharide. as well as being the first structure described containing 5-O-Me-Kdn. This Kdn-containing polysaccharide is not present in the wild-type strain HH103, which produces a 3-deoxy-D-manno-2-octulosonic acid (Kdo)-rich polysaccharide. We conclude that it is likely that the appearance of this new Kdn-containing polysaccharide is a consequence of the mutation.
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页码:585 / 594
页数:10
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共 37 条
[2]  
BERINGER JE, 1974, J GEN MICROBIOL, V84, P188
[3]   A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS [J].
BLAKENEY, AB ;
HARRIS, PJ ;
HENRY, RJ ;
STONE, BA .
CARBOHYDRATE RESEARCH, 1983, 113 (02) :291-299
[4]   C-13 NUCLEAR MAGNETIC-RESONANCE DATA FOR OLIGOSACCHARIDES [J].
BOCK, K ;
PEDERSEN, C ;
PEDERSEN, H .
ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1984, 42 :193-225
[5]   NATURAL ABUNDANCE N-15 NMR BY ENHANCED HETERONUCLEAR SPECTROSCOPY [J].
BODENHAUSEN, G ;
RUBEN, DJ .
CHEMICAL PHYSICS LETTERS, 1980, 69 (01) :185-189
[6]   DANTE-Z - A NEW APPROACH FOR ACCURATE FREQUENCY-SELECTIVITY USING HARD PULSES [J].
BOUDOT, D ;
CANET, D ;
BRONDEAU, J ;
BOUBEL, JC .
JOURNAL OF MAGNETIC RESONANCE, 1989, 83 (02) :428-439
[7]   PSEUDO-3D NMR OF PROTEINS WITH SELECTIVE EXCITATION BY DANTE-Z - ONE-DIMENSIONAL TOCSY-NOESY EXPERIMENT [J].
BOUDOT, D ;
ROUMESTAND, C ;
TOMA, F ;
CANET, D .
JOURNAL OF MAGNETIC RESONANCE, 1990, 90 (01) :221-227
[8]   HETEROGENEITY OF RHIZOBIUM LIPOPOLYSACCHARIDES [J].
CARLSON, RW .
JOURNAL OF BACTERIOLOGY, 1984, 158 (03) :1012-1017
[9]   STRUCTURES OF THE OLIGOSACCHARIDES OBTAINED FROM THE CORE REGIONS OF THE LIPOPOLYSACCHARIDES OF BRADYRHIZOBIUM-JAPONICUM 61A101C AND ITS SYMBIOTICALLY DEFECTIVE LIPOPOLYSACCHARIDE MUTANT, JS']JS314 [J].
CARLSON, RW ;
KRISHNAIAH, BS .
CARBOHYDRATE RESEARCH, 1992, 231 :205-219