The interaction of Staphylococcus aureus bi-component γ-hemolysins and leucocidins with cells and lipid membranes

被引:73
作者
Ferreras, M
Höper, F
Dalla Serra, M
Colin, DA
Prévost, G
Menestrina, G
机构
[1] CNR, ITC, Ctr fis Stati Aggregati, I-38050 Povo, Trento, Italy
[2] Univ Strasbourg, Fac Med, Inst Bacteriol, LTAB,UPRES EA 1318, F-67000 Strasbourg, France
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1998年 / 1414卷 / 1-2期
关键词
staphylococcal leucotoxin; hemolysis; toxin pore; phospholipid bilayer; Fourier-transform infrared spectroscopy; unilamellar vesicle; calcein release; Bi-component action; (Staphylococcus aureus);
D O I
10.1016/S0005-2736(98)00160-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Staphylococcus aureus gamma-hemolysins (HlgA, HlgB and HlgC) and Panton-Valentine leucocidins (LukS-PV and LukF-PV) are bi-component toxins forming a protein family with some relationship to alpha-toxin. Active toxins are couples formed by taking one protein from each of the two subfamilies of the S-components (LukS-PV, HlgA and HlgC) and the F-components (LukF-PV and HlgB). We compared the mode of action of the six possible couples on leukocytes, red blood cells and model lipid membranes. All couples were leucotoxic on human monocytes, whereas only four couples (HlgA+HlgB, HlgC+HlgB, LukS-PV+HlgB and HlgA+LukF-PV) were hemolytic. Toxins HlgA+HlgB and HlgC+HlgB were also able to induce permeabilisation of model membranes by forming pores via oligomerisation. The presence of membrane-bound aggregates, the smallest and most abundant of which had molecular weight and properties similar to that formed by alpha-toxin, was detected by SDS-PACE, By infrared spectroscopy in the attenuated total reflection configuration (FTIR-ATR), the secondary structure of both components and of the aggregate were determined to be predominantly beta-sheet and turn with small variations among different toxins. Polarisation experiments indicated that the structure of the membrane complex was compatible with the formation of a beta-barrel oriented perpendicularly to the plane of the membrane, similar to that of porins. The couple LukS-PV+LukF-PV was leucotoxic, but not hemolytic. When challenged against model membranes it was able to bind to the lipid vesicles and to form the aggregate with the beta-barrel structure, but not to increase calcein permeability. Thus, the pore-forming effect correlated with the hemolytic, but not with the complete leucotoxic activity of these toxins, suggesting that other mechanisms, like the interaction with endogenous cell proteins, might also play a role in their pathogenic action. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:108 / 126
页数:19
相关论文
共 41 条
[1]   Membrane permeabilizing activity of pathogenesis-related protein linusitin from flax seed [J].
Anzlovar, S ;
Dalla Serra, M ;
Dermastia, M ;
Menestrina, G .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (07) :610-617
[2]   QUANTITATIVE STUDIES OF THE STRUCTURE OF PROTEINS IN SOLUTION BY FOURIER-TRANSFORM INFRARED-SPECTROSCOPY [J].
ARRONDO, JLR ;
MUGA, A ;
CASTRESANA, J ;
GONI, FM .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1993, 59 (01) :23-56
[3]   The infrared dichroism of transmembrane helical polypeptides [J].
Axelsen, PH ;
Kaufman, BK ;
McElhaney, RN ;
Lewis, RNAH .
BIOPHYSICAL JOURNAL, 1995, 69 (06) :2770-2781
[4]   Staphylococcal alpha-toxin, streptolysin-O, and Escherichia coli hemolysin: Prototypes of pore-forming bacterial cytolysins [J].
Bhakdi, S ;
Bayley, H ;
Valeva, A ;
Walev, I ;
Walker, B ;
Weller, U ;
Kehoe, M ;
Palmer, M .
ARCHIVES OF MICROBIOLOGY, 1996, 165 (02) :73-79
[5]   EXAMINATION OF THE SECONDARY STRUCTURE OF PROTEINS BY DECONVOLVED FTIR SPECTRA [J].
BYLER, DM ;
SUSI, H .
BIOPOLYMERS, 1986, 25 (03) :469-487
[6]   PORE-FORMING AND HEMOLYTIC PROPERTIES OF THE GARDNERELLA-VAGINALIS CYTOLYSIN [J].
CAUCI, S ;
MONTE, R ;
ROPELE, M ;
MISSERO, C ;
NOT, T ;
QUADRIFOGLIO, F ;
MENESTRINA, G .
MOLECULAR MICROBIOLOGY, 1993, 9 (06) :1143-1155
[7]   Determination of molecular order in supported lipid membranes by internal reflection Fourier transform infrared spectroscopy [J].
Citra, MJ ;
Axelsen, PH .
BIOPHYSICAL JOURNAL, 1996, 71 (04) :1796-1805
[8]   INTERACTION OF THE 2 COMPONENTS OF LEUKOCIDIN FROM STAPHYLOCOCCUS-AUREUS WITH HUMAN POLYMORPHONUCLEAR LEUKOCYTE MEMBRANES - SEQUENTIAL BINDING AND SUBSEQUENT ACTIVATION [J].
COLIN, DA ;
MAZURIER, I ;
SIRE, S ;
FINCKBARBANCON, V .
INFECTION AND IMMUNITY, 1994, 62 (08) :3184-3188
[9]   THE GAMMA-HEMOLYSIN LOCUS OF STAPHYLOCOCCUS-AUREUS COMPRISES 3 LINKED GENES, 2 OF WHICH ARE IDENTICAL TO THE GENES FOR THE F AND S COMPONENTS OF LEUKOCIDIN [J].
COONEY, J ;
KIENLE, Z ;
FOSTER, TJ ;
OTOOLE, PW .
INFECTION AND IMMUNITY, 1993, 61 (02) :768-771
[10]  
Couppie P, 1997, ANN DERMATOL VENER, V124, P740