Functional role of position 22 in the homeodomain of Brn-3 transcription factors

被引：8

作者：

Dawson, SJ ^{[1
]}

Palmer, RD ^{[1
]}

Morris, PJ ^{[1
]}

Latchman, DS ^{[1
]}

机构：

[1] UCL, Sch Med, Windeyer Inst Med Sci, Dept Mol Pathol, London W1P 6DB, England

来源：

NEUROREPORT | 1998年 / 9卷 / 10期

关键词：

Brn-3; POU family; protein-protein interaction; transcriptional activation/repression; Vmw65;

D O I：

10.1097/00001756-199807130-00029

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE Brn-3a POU family transcription factor activates a number of neuronal promoters which are repressed by the closely related Brn-3b factor. Although transcriptional activation by mutant forms of Brn-3a/Brn-3b can occur with a number of different amino acids at position 22 of the POU homeodomain, an isoleucine at this position is of critical importance for transcriptional repression. In addition, this isoleucine residue can mediate an interaction with the herpes simplex virus Vmw65 regulatory protein whereas the valine found in Brn-3a cannot do so. Thus, position 22 in the homeodomain plays a critical role in the function of neuronally expressed POU factors by controlling their interaction with viral and cellular co-activator/co-repressor proteins. NeuroReport 9: 2305-2309 (C) 1998 Rapid Science Ltd.

引用

页码：2305 / 2309

页数：5

共 25 条

[1] A PROCEDURE TO STANDARDIZE CAT REPORTER GENE ASSAY [J].

ABKEN, H ;

REIFENRATH, B .

NUCLEIC ACIDS RESEARCH, 1992, 20 (13) :3527-3527

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] ACTIVATION OF THE ALPHA-INTERNEXIN PROMOTER BY THE BRN-3A TRANSCRIPTION FACTOR IS DEPENDENT ON THE N-TERMINAL REGION OF THE PROTEIN [J].

BUDHRAMMAHADEO, V ;

MORRIS, PJ ;

LAKIN, ND ;

THEIL, T ;

CHING, GY ;

LILLYCROP, KA ;

MOROY, T ;

LIEM, RKH ;

LATCHMAN, DS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (06) :2853-2858

[4] A single amino acid change converts an inhibitory transcription factor into an activator [J].

Dawson, SJ ;

Morris, PJ ;

Latchman, DS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (20) :11631-11633

[5] ASSOCIATION BETWEEN X-LINKED MIXED DEAFNESS AND MUTATIONS IN THE POU DOMAIN GENE POU3F4 [J].

DEKOK, YJM ;

VANDERMAAREL, SM ;

BITNERGLINDZICZ, M ;

HUBER, I ;

MONACO, AP ;

MALCOLM, S ;

PEMBREY, ME ;

ROPERS, HH ;

CREMERS, FPM .

SCIENCE, 1995, 267 (5198) :685-688

[6] A GENETIC PATHWAY FOR THE DEVELOPMENT OF THE CAENORHABDITIS-ELEGANS HSN MOTOR NEURONS [J].

DESAI, C ;

GARRIGA, G ;

MCINTIRE, SL ;

HORVITZ, HR .

NATURE, 1988, 336 (6200) :638-646

[7] Role of transcription factors Brn-3.1 and Brn-3.2 in auditory and visual system development [J].

Erkman, L ;

McEvilly, RJ ;

Luo, L ;

Ryan, AK ;

Hooshmand, F ;

OConnell, SM ;

Keithley, EM ;

Rapaport, DH ;

Ryan, AF ;

Rosenfeld, MG .

NATURE, 1996, 381 (6583) :603-606

[8] BRN-3.0 - A POU-DOMAIN PROTEIN EXPRESSED IN THE SENSORY, IMMUNE, AND ENDOCRINE SYSTEMS THAT FUNCTIONS ON ELEMENTS DISTINCT FROM KNOWN OCTAMER MOTIFS [J].

GERRERO, MR ;

MCEVILLY, RJ ;

TURNER, E ;

LIN, CR ;

OCONNELL, S ;

JENNE, KJ ;

HOBBS, MV ;

ROSENFELD, MG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (22) :10841-10845

[9]

Gorman C., 1985, DNA CLONING PRACTICA, V1, P143

[10] CRYSTAL-STRUCTURE OF THE OCT-1 POU DOMAIN BOUND TO AN OCTAMER SITE - DNA RECOGNITION WITH TETHERED DNA-BINDING MODULES [J].

KLEMM, JD ;

ROULD, MA ;

AURORA, R ;

HERR, W ;

PABO, CO .

CELL, 1994, 77 (01) :21-32

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