Conserved Eukaryotic Fusogens Can Fuse Viral Envelopes to Cells

被引：54

作者：

Avinoam, Ori ^{[1
]}

Fridman, Karen ^{[1
]}

Valansi, Clari ^{[1
]}

Abutbul, Inbal ^{[2
,3
]}

Zeev-Ben-Mordehai, Tzviya ^{[4
]}

Maurer, Ulrike E. ^{[5
]}

Sapir, Amir ^{[1
]}

Danino, Dganit ^{[2
,3
]}

Gruenewald, Kay ^{[4
,5
]}

White, Judith M. ^{[6
]}

Podbilewicz, Benjamin ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel

[2] Technion Israel Inst Technol, Dept Biotechnol & Food Engn, IL-32000 Haifa, Israel

[3] Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, IL-32000 Haifa, Israel

[4] Univ Oxford, Oxford Particle Imaging Ctr, Div Struct Biol, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England

[5] Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Germany

[6] Univ Virginia, Dept Cell Biol, Charlottesville, VA 22908 USA

来源：

SCIENCE | 2011年 / 332卷 / 6029期

基金：

英国惠康基金; 以色列科学基金会;

关键词：

VESICULAR STOMATITIS-VIRUS; MEMBRANE-FUSION; C-ELEGANS; GLYCOPROTEIN; MECHANISMS; EFF-1; PROTEINS;

D O I：

10.1126/science.1202333

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Caenorhabditis elegans proteins AFF-1 and EFF-1 [C. elegans fusion family (CeFF) proteins] are essential for developmental cell-to-cell fusion and can merge insect cells. To study the structure and function of AFF-1, we constructed vesicular stomatitis virus (VSV) displaying AFF-1 on the viral envelope, substituting the native fusogen VSV glycoprotein. Electron microscopy and tomography revealed that AFF-1 formed distinct supercomplexes resembling pentameric and hexameric "flowers" on pseudoviruses. Viruses carrying AFF-1 infected mammalian cells only when CeFFs were on the target cell surface. Furthermore, we identified fusion family (FF) proteins within and beyond nematodes, and divergent members from the human parasitic nematode Trichinella spiralis and the chordate Branchiostoma floridae could also fuse mammalian cells. Thus, FF proteins are part of an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle.

引用

页码：589 / 592

页数：4

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