Near IR emitting isothiocyanato-substituted fluorophores: their synthesis and bioconjugation to monoclonal antibodies

被引：26

作者：

Duan, WB ^{[1
]}

Smith, K

Savoie, H

Greenman, J

Boyle, RW

机构：

[1] Univ Hull, Dept Chem, Kingston Upon Hull HU6 7RX, N Humberside, England

[2] Univ Hull, Clin Biosci Inst, Kingston Upon Hull HU6 7RX, N Humberside, England

[3] Univ Hull, Postgrad Med Sch, Kingston Upon Hull HU6 7RX, N Humberside, England

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2005年 / 3卷 / 13期

基金：

英国惠康基金;

关键词：

D O I：

10.1039/b504334d

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Two near IR emitting fluorophores, based on the phthalocyanine and naphthalocyanine chromophores, which also bear a single isothiocyanato group suitable for conjugation to proteins are reported; their utility as luminescent probes is demonstrated by conjugation to monoclonal antibodies and the ability of these conjugates to selectively bind cells bearing the relevant antigen.

引用

页码：2384 / 2386

页数：3

共 14 条

[1] Poly(decyl methacrylate)-based fluorescent PEBBLE swarm nanosensors for measuring dissolved oxygen in biosamples [J].

Cao, YF ;

Koo, YEL ;

Kopelman, R .

ANALYST, 2004, 129 (08) :745-750

[2] Isothiocyanatoporphyrins, useful intermediates for the conjugation of porphyrins with biomolecules and solid supports [J].

Clarke, OJ ;

Boyle, RW .

CHEMICAL COMMUNICATIONS, 1999, (21) :2231-2232

[3]

Cook M. J., 1998, J CHEM SOC P1, P2453

[4] Long-wavelength fluorophores:: new trends in their analytical use [J].

Gómez-Hens, A ;

Aguilar-Caballos, MP .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2004, 23 (02) :127-136

[5] Strategies for selective delivery of photodynamic sensitisers to biological targets [J].

Hudson, R ;

Boyle, RW .

JOURNAL OF PORPHYRINS AND PHTHALOCYANINES, 2004, 8 (07) :954-975

[6] The development and characterisation of porphyrin isothiocyanate-monoclonal antibody conjugates for photoimmunotherapy [J].

Hudson, R ;

Carcenac, M ;

Smith, K ;

Madden, L ;

Clarke, OJ ;

Pèlegrin, A ;

Greenman, J ;

Boyle, RW .

BRITISH JOURNAL OF CANCER, 2005, 92 (08) :1442-1449

[7] PHOTODYNAMIC PROPERTIES OF NAPHTHOSULFOBENZOPORPHYRAZINES, NOVEL ASYMMETRIC, AMPHIPHILIC PHTHALOCYANINE DERIVATIVES [J].

MARGARON, P ;

LANGLOIS, R ;

VANLIER, JE ;

GASPARD, S .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1992, 14 (03) :187-199

[8] SYNTHESIS AND PHOTOPROPERTIES OF DIAMAGNETIC OCTABUTOXYPHTHALOCYANINES WITH DEEP RED OPTICAL ABSORBENCY [J].

RIHTER, BD ;

KENNEY, ME ;

FORD, WE ;

RODGERS, MAJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (22) :8064-8070

[9] PHOTOCHROMIC REACTIONS INVOLVING PALLADIUM(II) OCTABUTOXYNAPHTHALOCYANINE AND MOLECULAR-OXYGEN [J].

RIHTER, BD ;

KENNEY, ME ;

FORD, WE ;

RODGERS, MAJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (18) :8146-8152

[10]

Sutton JM, 2000, J PORPHYR PHTHALOCYA, V4, P655, DOI 10.1002/1099-1409(200011)4:7<655::AID-JPP245>3.0.CO

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