GlycoChip: multiarray for the study of carbohydrate-binding proteins

被引：60

作者：

Galanina, OE

Mecklenburg, M

Nifantiev, NE

Pazynina, GV

Bovin, NV

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Interact GmbH, Ulm, Germany

[3] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow, Russia

来源：

LAB ON A CHIP | 2003年 / 3卷 / 04期

关键词：

D O I：

10.1039/b305963d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Biotinylated glycoconjugates which were designed as oligosaccharides attached to 30 kDa polyacrylamide were coated on a microarray platform XNAonGOLD(TM), which was developed earlier for an oligonucleotide assay. The specificity of antibodies to carbohydrate antigens was analyzed using the glyco-microarray. Comparison of the obtained results with those of common 96-well plate ELISA completely coincided with the found antibody specificities. However, parameters such as the analytical sensitivity of the method and the amount of biotinylated material coated on the microarray platform proved to be worse than expected.

引用

页码：260 / 265

页数：6

共 17 条

[1] A streptavidin surface on planar glass substrates for the detection of biomolecular interaction [J].