Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes

被引：50

作者：

Michalet, X

Lacoste, TD

Weiss, S

机构：

[1] Lawrence Berkeley Lab, Div Mat Sci, Berkeley, CA 94720 USA

[2] Lawrence Berkeley Lab, Phys Biosci Div, Berkeley, CA 94720 USA

来源：

METHODS | 2001年 / 25卷 / 01期

关键词：

superresolution; diffraction limit; fluorescence; microscopy; confocal; two-photon; single molecule; TransFluoSphere; semiconductor nanocrystal; quantum dot;

D O I：

10.1006/meth.2001.1218

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An ultrahigh-resolution colocalization method based on the simultaneous acquisition and analysis of spectrally separated images of the excitation point-spread function of point-like fluorescent probes Is reviewed. It is shown that molecular distances can be measured with accuracy better than 10 nm using conventional far-field optics. A detailed account of the methodology, theoretical considerations, signal processing, and data fitting algorithms is given. (C) 2001 Academic Press.

引用

页码：87 / 102

页数：16

共 72 条

[1] Semiconductor clusters, nanocrystals, and quantum dots [J].