Phenanthroline-Cu complex-mediated chemiluminescence of DNA and its potential use in antioxidation evaluation

被引：52

作者：

Ma, WJ ^{[1
]}

Cao, EH ^{[1
]}

Zhang, J ^{[1
]}

Qin, JF ^{[1
]}

机构：

[1] Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1998年 / 44卷 / 01期

基金：

中国国家自然科学基金;

关键词：

DNA damage; antioxidants; phenanthroline-CU2+ complex; oxidative stress; reactive oxygen species; chemiluminescence;

D O I：

10.1016/S1011-1344(98)00120-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The chemiluminescence (CL) concomitant with phen-Cu2+/ascorbate/H2O2-induced DNA damage has been studied. The emission intensity increases linearly with increasing DNA concentration. The emission spectrum has a maximal wavelength at about 410 nm. The luminescence is inhibited by histone in a histone concentration-dependent manner. The CL is characteristic of guanine. Of all common kinds of bases and nucleotides, only guanine or guanine nucleotides can give rise to luminescence. The possibility of using the luminescence as a means of studying antioxidation related to DNA damage is discussed. Several kinds of well-defined antioxidants have been used as testing reagents and show that this method can not only evaluate the antioxidative effect but also distinguish different types of antioxidants. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：63 / 68

页数：6

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