Identification of biologically active and inactive human uroguanylins in plasma and urine and their increases in renal insufficiency

被引:58
作者
Nakazato, M [1 ]
Yamaguchi, H
Kinoshita, H
Kangawa, K
Matsuo, H
Chino, N
Matsukura, S
机构
[1] MIYAZAKI MED COLL,DEPT INTERNAL MED 1,MIYAZAKI 88916,JAPAN
[2] NATL CARDIOVASC CTR,RES INST,SUITA,OSAKA 565,JAPAN
[3] PEPTIDE INST INC,MINO,OSAKA 562,JAPAN
关键词
D O I
10.1006/bbrc.1996.0447
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Uroguanylin, a 16-amino acid peptide, is an endogenous activator of intestinal and possibly renal guanylate cyclase C (GC-C). Using two synthetic topological isoforms of human uroguanylin, one bioactive, the other inactive, we prepared two antisera specific for the individual isoforms and developed sensitive radioimmunoassays (RIAs). The respective plasma concentrations of the bioactive and inactive uroguanylins in the normal individuals tested were 5.0 +/- 0.3 fmol/ml (mean +/- SE) and 1.6 +/- 0.1 fmol/ml. These concentrations increased in chronic renal failure (CRF). The major endogenous uroguanylin molecule in normal human urine was 16 amino acids long, whereas in the plasma and urine of CRF patients the major molecule was 10-kDa prouro-guanylin. The RIAs established are promising tools for clarifying the physiological functions and pathophysiological implications of uroguanylin in water and electrolyte homeostasis. (C) 1996 Academic Press, Inc.
引用
收藏
页码:586 / 593
页数:8
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