In vitro thermal therapy of AT-1 Dunning prostate tumours

被引:40
作者
Bhowmick, S
Coad, JE
Swanlund, DJ
Bischof, JC
机构
[1] Univ Minnesota, Dept Mech Engn, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA
[3] Univ Massachusetts, Dept Mech Engn, N Dartmouth, MA 02747 USA
[4] W Virginia Univ, Sch Med, RCB Hlth Sci Ctr N 2189, Dept Pathol, Morgantown, WV 26508 USA
关键词
thermal therapy; Dunning AT-1 rat prostate tumour; cell injury; necrosis; apoptosis;
D O I
10.1080/0265673031000111932
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To advance the utility of prostate thermal therapy, this study investigated the thermal thresholds (temperature-time) for prostate tissue destruction in vitro. The AT-1 Dunning prostate tumour model was chosen for the study. Three hundred micron thick sections were subjected to controlled temperature-time heating, which ranged from low (40degreesC, 15 min) to high thermal exposures (70degreesC, 2 min) (n = 6). After subsequent tissue culture at 37degreesC, the sections were evaluated for tissue injury at 3, 24 and 72 h by two independent methods: histology and dye uptake. A graded increase in injury was identified between the low and high thermal exposures. Maximum histologic injury occurred above 70degreesC, 1 min with >95% of the tissue area undergoing significant cell injury and coagulative necrosis. The control and 40degreesC, 15 min sections showed histologic evidence of apoptosis following 24 and 72 h in culture. Similar signs of apoptosis were minimal or absent at higher thermal histories. Vital-dye uptake quantitatively confirmed complete cell death after 70degreesC, 2 min. Using the dye data, Arrhenius analysis showed an apparent breakpoint at 50degreesC, with activation energies of 135.8 kcal/mole below and 4.7 kcal/mole above the threshold after 3 h in culture. These results can be used as a conservative benchmark for thermal injury in the cancerous prostate. Further characterization of the response to thermal therapy in an animal model and in human tissues will be important in establishing the efficacy of the procedure.
引用
收藏
页码:73 / 92
页数:20
相关论文
共 57 条
[2]  
BERNDT WO, 1987, NEPHROTOXICITY EXPT, P301
[3]   Thermal therapy of prostate tumor tissue in the dorsal skin flap chamber [J].
Bhowmick, S ;
Hoffmann, NE ;
Bischof, JC .
MICROVASCULAR RESEARCH, 2002, 64 (01) :170-173
[4]   Supraphysiological thermal injury in Dunning AT-1 prostate tumor cells [J].
Bhowmick, S ;
Swanlund, DJ ;
Bischof, JC .
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 2000, 122 (01) :51-59
[5]   Evaluation of thermal therapy in a prostate cancer model using a wet electrode radiofrequency probe [J].
Bhowmick, S ;
Swanlund, DJ ;
Coad, JE ;
Lulloff, L ;
Hoey, MF ;
Bischof, JC .
JOURNAL OF ENDOUROLOGY, 2001, 15 (06) :629-640
[6]   Cryosurgery of dunning AT-1 rat prostate tumor: Thermal, biophysical, and viability response at the cellular and tissue level [J].
Bischof, JC ;
Smith, D ;
Pazhayannur, PV ;
Manivel, C ;
Hulbert, J ;
Roberts, KP .
CRYOBIOLOGY, 1997, 34 (01) :42-69
[7]   DYNAMICS OF CELL-MEMBRANE PERMEABILITY CHANGES AT SUPRAPHYSIOLOGICAL TEMPERATURES [J].
BISCHOF, JC ;
PADANILAM, J ;
HOLMES, WH ;
EZZELL, RM ;
LEE, RC ;
TOMPKINS, RG ;
YARMUSH, ML ;
TONER, M .
BIOPHYSICAL JOURNAL, 1995, 68 (06) :2608-2614
[8]   TIME-TEMPERATURE ANALYSIS OF CELL KILLING OF BHK CELLS HEATED AT TEMPERATURES IN THE RANGE OF 43.5-DEGREES-C TO 57.0-DEGREES-C [J].
BORRELLI, MJ ;
THOMPSON, LL ;
CAIN, CA ;
DEWEY, WC .
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1990, 19 (02) :389-399
[9]  
BUCKLEY IK, 1972, LAB INVEST, V26, P201
[10]   LAPAROSCOPIC ABLATION OF LIVER ADENOMA BY RADIOFREQUENCY ELECTROCAUTHERY [J].
BUSCARINI, L ;
ROSSI, S ;
FORNARI, F ;
DISTASI, M ;
BUSCARINI, E .
GASTROINTESTINAL ENDOSCOPY, 1995, 41 (01) :68-70