Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

被引：21

作者：

Wahyudi, AT ^{[1
]}

Takeyama, H ^{[1
]}

Matsunaga, T ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2001年 / 91-3卷 / 1-9期

关键词：

transposon mutagenesis; mini-Tn5; Magnetospirillum magneticum AMB-1; inverse polymerase chain reaction; bacterial magnetic particles; sequence analysis;

D O I：

10.1385/ABAB:91-93:1-9:147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 x 10(-7) per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

引用

页码：147 / 154

页数：8

共 25 条

[1]

Berg D. E., 1989, M ohile DNA, P185

[2] ISOLATION AND PURE CULTURE OF A FRESHWATER MAGNETIC SPIRILLUM IN CHEMICALLY DEFINED MEDIUM [J].

BLAKEMORE, RP ;

MARATEA, D ;

WOLFE, RS .

JOURNAL OF BACTERIOLOGY, 1979, 140 (02) :720-729

[3] Genome sequence of the nematode C-elegans:: A platform for investigating biology [J].

不详 .

SCIENCE, 1998, 282 (5396) :2012-2018

[4] CLONING AND SEQUENCING OF PSEUDOMONAS GENES DETERMINING SODIUM DODECYL-SULFATE BIODEGRADATION [J].

DAVISON, J ;

BRUNEL, F ;

PHANOPOULOS, A ;

PROZZI, D ;

TERPSTRA, P .

GENE, 1992, 114 (01) :19-24

[5] THE USE OF TRANSPOSON TN5 MUTAGENESIS IN THE RAPID GENERATION OF CORRELATED PHYSICAL AND GENETIC MAPS OF DNA SEGMENTS CLONED INTO MULTICOPY PLASMIDS - A REVIEW [J].

DEBRUIJN, FJ ;

LUPSKI, JR .

GENE, 1984, 27 (02) :131-149

[6] The complete genome of the hyperthermophilic bacterium Aquifex aeolicus [J].

Deckert, G ;

Warren, PV ;

Gaasterland, T ;

Young, WG ;

Lenox, AL ;

Graham, DE ;

Overbeek, R ;

Snead, MA ;

Keller, M ;

Aujay, M ;

Huber, R ;

Feldman, RA ;

Short, JM ;

Olsen, GJ ;

Swanson, RV .

NATURE, 1998, 392 (6674) :353-358

[7] MINI-TN5 TRANSPOSON DERIVATIVES FOR INSERTION MUTAGENESIS, PROMOTER PROBING, AND CHROMOSOMAL INSERTION OF CLONED DNA IN GRAM-NEGATIVE EUBACTERIA [J].

DELORENZO, V ;

HERRERO, M ;

JAKUBZIK, U ;

TIMMIS, KN .

JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6568-6572

[8] Molecular basis of symbiosis between Rhizobium and legumes [J].

Freiberg, C ;

Fellay, R ;

Bairoch, A ;

Broughton, WJ ;

Rosenthal, A ;

Perret, X .

NATURE, 1997, 387 (6631) :394-401

[9] TRANSPOSON VECTORS CONTAINING NON-ANTIBIOTIC RESISTANCE SELECTION MARKERS FOR CLONING AND STABLE CHROMOSOMAL INSERTION OF FOREIGN GENES IN GRAM-NEGATIVE BACTERIA [J].

HERRERO, M ;

DELORENZO, V ;

TIMMIS, KN .

JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6557-6567

[10] cis-trans isomerization of unsaturated fatty acids: Cloning and sequencing of the cti gene from Pseudomonas putida P8 [J].

Holtwick, R ;

Meinhardt, F ;

Keweloh, H .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (11) :4292-4297

← 1 2 3 →