Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis

被引：21

作者：

Wahyudi, AT ^{[1
]}

Takeyama, H ^{[1
]}

Matsunaga, T ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2001年 / 91-3卷 / 1-9期

关键词：

transposon mutagenesis; mini-Tn5; Magnetospirillum magneticum AMB-1; inverse polymerase chain reaction; bacterial magnetic particles; sequence analysis;

D O I：

10.1385/ABAB:91-93:1-9:147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 x 10(-7) per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

引用

页码：147 / 154

页数：8

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