Resistance to linezolid: Characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci

被引:239
作者
Prystowsky, J
Siddiqui, F
Chosay, J
Shinabarger, DL
Millichap, J
Peterson, LR
Noskin, GA
机构
[1] Northwestern Univ, Sch Med, Div Infect Dis, Dept Med, Chicago, IL 60611 USA
[2] Northwestern Univ, Sch Med, Dept Pathol, Clin Microbiol Div, Chicago, IL 60611 USA
[3] NW Mem Hosp, Prevent Epictr, Chicago, IL 60611 USA
[4] Pharmacia Corp, Kalamazoo, MI USA
关键词
D O I
10.1128/AAC.45.7.2154-2156.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To assess the potential for emergence of resistance during the use of linezolid, we tested 10 clinical isolates of vancomycin-resistant enterococci (VRE) (four Enterococcus faecalis, five Enterococcus faecium, and one Enterococcus gallinarum) as well as a vancomycin-susceptible control (ATCC 29212) strain of E. faecalis, The enterococci were exposed to doubling dilutions of linezolid for 12 passes. After the final passage, the linezolid plate growing VRE contained a higher drug concentration with E. faecalis than with E. faecium. DNA sequencing of the 23S rRNA genes revealed that linezolid resistance in three E. faecalis isolates was associated with a guanine to uracil transversion at bp 2576, while the one E. faecium isolate for which the MIC was 16 mug/ml contained a guanine to adenine transition at bp 2505.
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页码:2154 / 2156
页数:3
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