Induction of cytokines in a human colon epithelial cell line by Shiga toxin 1 (Stx1) and Stx2 but not by non-toxic mutant Stx1 which lacks N-glycosidase activity

被引：59

作者：

Yamasaki, C ^{[1
]}

Natori, Y ^{[1
]}

Zeng, XT ^{[1
]}

Ohmura, M ^{[1
]}

Yamasaki, S ^{[1
]}

Takeda, Y ^{[1
]}

Natori, Y ^{[1
]}

机构：

[1] Int Med Ctr Japan, Res Inst, Dept Clin Pharmacol, Shinjuku Ku, Tokyo 1628655, Japan

来源：

FEBS LETTERS | 1999年 / 442卷 / 2-3期

关键词：

Shiga toxin; cytokine; interleukin-8; RNA N-glycosidase; colon epithelial cell; Shiga toxin-producing Escherichia coli;

D O I：

10.1016/S0014-5793(98)01667-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stx1 and Stx2 produced by Shiga toxin-producing Escherichia coli are cytotoxic due to their N-glycosidase activity on 28S rRNA, In this study, we have shown that proinflammatory cytokine mRNAs, especially IL-8, were induced by Stx1 and Stx2 in Caco-2 cells. A non-toxic mutant of Sts1 which lacks N-glycosidase activity did not induce cytokine mRNAs, IL-8 production at the protein level was enhanced by Stx1 and Stx2, but not by the mutant Stx1, These results demonstrate that Shiga toxins induce expression and synthesis of cytokines in Caco-2 cells and their N-glycosidase activity is essential for the induction. (C) 1999 Federation of European Biochemical Societies.

引用

页码：231 / 234

页数：4

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