Microphthalmia transcription factor - A sensitive and specific marker for malignant melanoma in cytologic specimens

被引:33
作者
Dorvault, CC
Weilbaecher, KN
Yee, H
Fisher, DE
Chiriboga, LA
Xu, Y
Chhieng, DC
机构
[1] Univ Alabama, Dept Pathol, Birmingham, AL 35249 USA
[2] Washington Univ, Div Oncol, St Louis, MO USA
[3] NYU Med Ctr, Dept Pathol, New York, NY 10016 USA
[4] Dana Farber Canc Inst, Div Pediat Oncol, Boston, MA 02115 USA
[5] Childrens Hosp, Boston, MA 02115 USA
来源
CANCER CYTOPATHOLOGY | 2001年 / 93卷 / 05期
关键词
microphthalmia transcription factor; melanoma; S-100; HMB-45; cytology;
D O I
10.1002/cncr.9049
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
BACKGROUND. The diagnosis of melanoma can be difficult because of shared cytomorphology with other malignant neoplasms. The most commonly used melanocytic markers, anti-S-100 protein and HMB-45 antigen, have limited specificity and sensitivity, respectively. Microphthalmia transcription factor (Mitf) is a nuclear transcription factor critical for the development and survival of melanocytes and has been shown as a sensitive and specific marker for melanoma in histologic specimens. METHODS. To evaluate the efficacy of Mitf as a marker for melanoma in cytologic preparations, 81 cell blocks from 44 patients with melanoma and 37 patients with nonmelanoma malignancies (29 patients with carcinoma, 4 patients with mesotheliomas, 2 patients with lymphoma, and 2 patients with islet cell tumors) were stained with monoclonal antibodies against Mitf (clone D5), S-100 protein, and HMB-45 antigen. The staining was evaluated blindly by three independent observers. The presence of nuclear staining for Mitf and cytoplasmic staining for S-100 protein or HMB-45 antigen in > 10% of tumor cells was considered positive staining for each antigen. RESULTS. Forty-four melanomas (100%), including all 3 spindle-cell melanomas, were positive for Mitf. All nonmelanoma neoplasms were negative with only one exception: One mammary carcinoma showed rare (< 10%), weak nuclear staining with Mitf. The sensitivity and specificity of Mitf as a marker for melanoma were both 100%, whereas the sensitivity of HMB-45 antigen was 90.4%, and the specificity of S-100 protein was 70.3%. CONCLUSIONS. Mitf is a sensitive and specific marker for malignant melanoma, including the spindle-cell variant, in cytologic specimens and may be superior to the current standard melanocytic markers, S-100 protein and HMB-45 antigen. (C) 2001 American Cancer Society.
引用
收藏
页码:337 / 343
页数:7
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