Recent advances in FRET: distance determination in protein-DNA complexes

被引：150

作者：

Hillisch, A

Lorenz, M

Diekmann, S

机构：

[1] EnTec GmbH, D-07745 Jena, Germany

[2] Inst Mol Biotechnol, D-07745 Jena, Germany

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2001年 / 11卷 / 02期

关键词：

D O I：

10.1016/S0959-440X(00)00190-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence resonance energy transfer (FRET) provides information on the distance between a donor and an acceptor dye in the range 10 to 100 Angstrom. Knowledge of the exact positions of some dyes with respect to nucleic acids now enables us to translate these data into precise structural information using molecular modeling. Advances in the preparation of dye-labeled nucleic acid molecules and in new techniques, such as the measurement of FRET in polyacrylamide gels or in vivo, will lead to an increasingly important role of FRET in structural and molecular biology.

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收藏

页码：201 / 207

页数：7

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