Enzyme precipitate coatings of lipase on polymer nanofibers

被引：12

作者：

An, Hyo Jin ^{[1
]}

Lee, Hye-Jin ^{[1
]}

Jun, Seung-Hyun ^{[1
]}

Hwang, Sang Youn ^{[1
]}

Kim, Byoung Chan ^{[2
]}

Kim, Kwanghee ^{[3
,4
]}

Lee, Kyung-Mi ^{[3
,4
]}

Oh, Min-Kyu ^{[1
]}

Kim, Jungbae ^{[1
]}

机构：

[1] Korea Univ, Dept Chem & Biol Engn, Seoul 136701, South Korea

[2] Korea Inst Sci & Technol, Environm Div, Seoul 136791, South Korea

[3] Korea Univ, Global Res Lab, Dept Biochem, Seoul 136701, South Korea

[4] Korea Univ, Div Brain, Korea Program Biomed Sci 21, Coll Med, Seoul 136701, South Korea

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2011年 / 34卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

Enzyme precipitate coatings; Electrospun polymer nanofibers; Ethanol dispersion; Lipase; Enzyme stabilization; WALLED CARBON NANOTUBES; MESOPOROUS SILICA; IMMOBILIZED ENZYMES; CATALYTIC ACTIVITY; PROTEIN DIGESTION; BIOFUEL CELLS; STABILITY; NANOPARTICLES; BIOCATALYSTS; ELECTROSPUN;

D O I：

10.1007/s00449-011-0534-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipase (LP) was immobilized on electrospun and ethanol-dispersed polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers (EtOH-NF) in the form of enzyme precipitate coatings (EPCs). LP precipitate coatings (EPCs-LP) were prepared in a three-step process, consisting of covalent attachment, LP precipitation, and crosslinking of precipitated LPs onto the covalently attached LPs via glutaraldehyde treatment. The LP precipitation was performed by adding various concentrations of ammonium sulfate (20-50%, w/v). EPCs-LP improved the LP activity and stability when compared to covalently attached LPs (CA-LP) and the enzyme coatings of LPs (EC-LP) without the LP precipitation. For example, the use of 40% (w/v) ammonium sulfate resulted in EPC40-LP with the highest activity, which was 4.0 and 3.6 times higher than those of CA-LP and EC-LP, respectively. After 165-day incubation under rigorous shaking at 200 rpm, the residual activities of EPC50-LP were 0.5 mu M/min mg of EtOH-NF, representing 113 and 75 times higher than those of CA-LP and EC-LP, respectively. When LP was partially purified via a simple ammonium sulfate precipitation and dialysis, both activities and stabilities of EC-LP and EPC-LP could be marginally improved. It is anticipated that the improved LP activity and stability in the form of EPCs would allow for their potential applications in various bioconversion processes such as biodiesel production and ibuprofen resolution.

引用

页码：841 / 847

页数：7

共 37 条

[1] Robust Trypsin Coating on Electrospun Polymer Nanofibers in Rigorous Conditions and Its Uses for Protein Digestion [J].

Ahn, Hye-Kyung ;

Kim, Byoung Chan ;

Jun, Seung-Hyun ;

Chang, Mun Seock ;

Lopez-Ferrer, Daniel ;

Smith, Richard D. ;

Gu, Man Bock ;

Lee, Sang-Won ;

Kim, Beom Soo ;

Kim, Jungbae .

BIOTECHNOLOGY AND BIOENGINEERING, 2010, 107 (06) :917-923

[2] Bioelectrochemical single-walled carbon nanotubes [J].

Azamian, BR ;

Davis, JJ ;

Coleman, KS ;

Bagshaw, CB ;

Green, MLH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (43) :12664-12665

[3]

Bognitzki M, 2001, ADV MATER, V13, P70, DOI 10.1002/1521-4095(200101)13:1<70::AID-ADMA70>3.3.CO

[4]

2-8

[5] THE ESTERASE PROFILE OF A LIPASE FROM CANDIDA-CYLINDRACEA [J].

BRAHIMIHORN, MC ;

GUGLIELMINO, ML ;

ELLING, L ;

SPARROW, LG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1042 (01) :51-54

[6] Noncovalent sidewall functionalization of single-walled carbon nanotubes for protein immobilization [J].

Chen, RJ ;

Zhang, YG ;

Wang, DW ;

Dai, HJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (16) :3838-3839

[7] Enzyme immobilization in MCM-41 molecular sieve [J].

Diaz, JF ;

Balkus, KJ .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1996, 2 (2-3) :115-126

[8] Activity of Candida rugosa lipase immobilized on γ-Fe2O3 magnetic nanoparticles [J].

Dyal, A ;

Loos, K ;

Noto, M ;

Chang, SW ;

Spagnoli, C ;

Shafi, KVPM ;

Ulman, A ;

Cowman, M ;

Gross, RA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (07) :1684-1685

[9] Miniature biofuel cells with improved stability under continuous operation [J].

Fischback, Michael B. ;

Youn, Jong Kyu ;

Zhao, Xueyan ;

Wang, Ping ;

Park, Hyun Gyu ;

Chang, Ho Nam ;

Kim, Jungbae ;

Ha, Su .

ELECTROANALYSIS, 2006, 18 (19-20) :2016-2022

[10] Lipase-catalyzed esterification [J].

Gandhi, NN ;

Patil, NS ;

Sawant, SB ;

Joshi, JB ;

Wangikar, PP ;

Mukesh, D .

CATALYSIS REVIEWS-SCIENCE AND ENGINEERING, 2000, 42 (04) :439-480

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