Dentin matrix protein 1 is predominantly expressed in chicken and rat osteocytes but not in osteoblasts

被引:222
作者
Toyosawa, S
Shintani, S
Fujiwara, T
Ooshima, T
Sato, A
Ijuhin, N
Komori, T
机构
[1] Osaka Univ, Grad Sch Dent, Dept Oral Pathol, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Dent, Dept Pedodont, Suita, Osaka 5650871, Japan
[3] Max Planck Inst Biol, Immungenet Abt, D-7400 Tubingen, Germany
[4] Osaka Univ, Grad Sch Med, Dept Mol Med, Suita, Osaka 5650871, Japan
[5] Japan Sci & Technol Corp, PRESTO, Osaka, Japan
关键词
dentin matrix protein 1; osteocyte; osteoblast; in situ hybridization; immunohistochemistry;
D O I
10.1359/jbmr.2001.16.11.2017
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Although osteocytes are the most abundant cells in bone, little is known about their function, and no specific marker protein for osteocytes has been described. Dentin matrix protein 1 (DMP1) is an acidic phosphoprotein expressed in tooth organ and bone. Our previous work showed that in the chicken, which is not capable of forming tooth, DMP1 messenger RNA (mRNA) is highly expressed in bone by Northern blot analysis. To clarify the significance of DMP1 expression in bone, the expression of DMP1 mRNA and its protein was examined in the chicken and rat. In the chicken, DMP1 mRNA was detected only in bone tissues and was localized in osteocytes and preosteocytes but not in osteoblasts. Similarly, in the rat, DMP1 mRNA was predominantly expressed in osteocytes and preosteocytes. in bone matrix but not in osteoblasts located at the bone surface. Antiserum. was raised against the peptide from rat DMP1, and the localization of DMP1 was examined by immunohistochemistry. In the development of bone, DMP1 was first detected in newly formed bone matrix after osteoblastic cells had been embedded within it. After the appearance of typical osteocytes, DMP1 was localized in the pericellular bone matrix of osteocytes, including their processes. These data show that DMP1 is a bone matrix protein specifically expressed in osteocytes. and preosteocytes and suggest that DMP1 plays a role in bone homeostasis because of its high calcium ion-binding capacity.
引用
收藏
页码:2017 / 2026
页数:10
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