Mutation of the zinc-binding metalloprotease motif affects Bacteroides fragilis toxin activity but does not affect propeptide processing

被引：24

作者：

Franco, AA

Buckwold, SL

Shin, JW

Ascon, M

Sears, CL

机构：

[1] Johns Hopkins Univ, Sch Med, Div Infect Dis, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Div Gastroenterol, Dept Med, Baltimore, MD 21205 USA

来源：

INFECTION AND IMMUNITY | 2005年 / 73卷 / 08期

关键词：

D O I：

10.1128/IAI.73.8.5273-5277.2005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To evaluate the role of the zinc-binding metalloprotease in Bacteroides fragilis toxin (BFT) processing and activity, the zinc-binding consensus sequences (H348, E349, H352, G355, H358, and M366) were mutated by site-directed-mutagenesis. Our results indicated that single point mutations in the zinc-binding metalloprotease motif do not affect BFT processing but do reduce or eliminate BFT biologic activity in vitro.

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页码：5273 / 5277

页数：5

相关论文

共 40 条

[1]

Bode W, 1996, ADV EXP MED BIOL, V389, P1

[2] Mutational analysis of the proteolytic domain of pregnancy-associated plasma protein-A (PAPP-A): classification as a metzincin [J].

Boldt, HB ;

Overgaard, MT ;

Laursen, LS ;

Weyer, K ;

Sottrup-Jensen, L ;

Oxvig, C .

BIOCHEMICAL JOURNAL, 2001, 358 (02) :359-367

[3] Bacteroides fragilis toxin exhibits polar activity on monolayers of human intestinal epithelial cells (T84 cells) in vitro [J].

Chambers, FG ;

Koshy, SS ;

Saidi, RF ;

Clark, DP ;

Moore, RD ;

Sears, CL .

INFECTION AND IMMUNITY, 1997, 65 (09) :3561-3570

[4] Identification of a third metalloprotease toxin gene in extraintestinal isolates of Bacteroides fragilis [J].

Chung, GT ;

Franco, AA ;

Wu, SG ;

Rhie, GE ;

Cheng, R ;

Oh, HB ;

Sears, CL .

INFECTION AND IMMUNITY, 1999, 67 (09) :4945-4949

[5] Molecular evolution of the pathogenicity island of enterotoxigenic Bacteroides fragilis strains [J].

Franco, AA ;

Cheng, RK ;

Chung, GT ;

Wu, SG ;

Oh, HB ;

Sears, CL .

JOURNAL OF BACTERIOLOGY, 1999, 181 (21) :6623-6633

[6] The Bacteroides fragilis pathogenicity island is contained in a putative novel conjugative transposon [J].

Franco, AA .

JOURNAL OF BACTERIOLOGY, 2004, 186 (18) :6077-6092

[7] Modulation of bft expression by the Bacteroides fragilis pathogenicity island and its flanking region [J].

Franco, AA ;

Cheng, RK ;

Goodman, A ;

Sears, CL .

MOLECULAR MICROBIOLOGY, 2002, 45 (04) :1067-1077

[8] Cloning and characterization of the Bacteroides fragilis metalloprotease toxin gene [J].

Franco, AA ;

Mundy, LM ;

Trucksis, M ;

Wu, SG ;

Kaper, JB ;

Sears, CL .

INFECTION AND IMMUNITY, 1997, 65 (03) :1007-1013

[9] Structural aspects of the metzincin clan of metalloendopeptidases [J].

Gomis-Rüth, FX .

MOLECULAR BIOTECHNOLOGY, 2003, 24 (02) :157-202

[10] PLASMID TRANSFER FROM ESCHERICHIA-COLI TO BACTEROIDES-FRAGILIS - DIFFERENTIAL EXPRESSION OF ANTIBIOTIC-RESISTANCE PHENOTYPES [J].

GUINEY, DG ;

HASEGAWA, P ;

DAVIS, CE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (22) :7203-7206

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