Liquid culture of the entomopathogenic nematod-bacterium-complex Heterorhabditis megidis Photorhabdus luminescens

被引：87

作者：

Ehlers, RU ^{[1
]}

Lunau, S ^{[1
]}

Krasomil-Osterfeld, K ^{[1
]}

Osterfeld, KH ^{[1
]}

机构：

[1] Univ Kiel, Inst Phytopathol, Dept Biotechnol & Biol Control, D-24223 Raisdorf, Germany

来源：

BIOCONTROL | 1998年 / 43卷 / 01期

关键词：

bioreactor liquid culture; entomopathogenic nematodes; phase variants; recovery;

D O I：

10.1023/A:1009965922794

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A process technology for production of the entomopathogenic biocontrol nematode-bacterium complex Heterorhabditis megidis/Photorhabdus luminescens in monoxenic liquid culture in laboratory scale bioreactors is described. Dauer juvenile yields varied between 21 and 68 million dauer juveniles/l medium. The maximum density was reached at 13 to 25 days after inoculation of Tt luminescens. The reason for the high variability in yield was identified. After the 23-h bacterial preculture the bioreactor is inoculated with nematode dauer juveniles which develop to self-fertilizing hermaphrodites. The exit from enduring dauer stages (recovery) was between 18 and 90% of the inoculum density. Low dauer juvenile recovery resulted in the development of two-generations within 20 to 25 days. In contrast, high dauer juvenile recovery led to a one-generation process terminated within 15 days. Factors influencing dauer recovery are still unknown.

引用

页码：77 / 86

页数：10