A restriction enzyme-based assay to distinguish between avian hemosporidians

被引：69

作者：

Beadell, JS

Fleischer, RC

机构：

[1] Smithsonian Inst, Natl Museum Nat Hist, Genet Program, Washington, DC 20008 USA

[2] Univ Maryland, Dept Biol, College Pk, MD 20742 USA

来源：

JOURNAL OF PARASITOLOGY | 2005年 / 91卷 / 03期

关键词：

D O I：

10.1645/GE-3412RN

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We describe a reliable and relatively inexpensive method for detecting and differentiating between the commonly studied avian blood parasite genera Haemoproteus, Plasmodium, and Leitcocytozooll. The assay takes advantage of a Haemoproteus-specific restriction site identified by sequencing full rnitochondrial genomes from two Haemoproteus and three Plosmodium lineages and an adjacent, genus-specific restriction site identified in Leucocytozoon spp. The assay was sensitive to simulated parasitemas of approximately 8 x 10(-6) per erythrocyte and was 100% accurate in differentiating between parasite genera isolated from a broad geographical and taxonomic sampling of infected hosts.

引用

页码：683 / 685

页数：3

共 24 条

[1]

Atkinson C.T., 1991, BIRD PARASITE INTERA, P19

[2] Wildlife disease and conservation in Hawaii: Pathogenicity of avian malaria (Plasmodium relictum) in experimentally infected Iiwi (Vestiaria coccinea) [J].