Development of a portable biosensor for screening neurotoxic agents in water samples

被引：35

作者：

Hildebrandt, Alain ^{[1
]}

Ribas, Jordi ^{[1
]}

Bragos, Ramon ^{[2
]}

Marty, Jean-Louis ^{[3
]}

Tresanchez, Marius ^{[1
]}

Lacorte, Silvia ^{[1
]}

机构：

[1] CSIC, IIQAB, Dept Environm Chem, ES-08034 Barcelona, Spain

[2] Univ Politecn Cataluna, Dept Elect Engn, ES-08034 Barcelona, Spain

[3] Univ Perpignan, Ctr Phytopharm, F-66860 Perpignan, France

来源：

TALANTA | 2008年 / 75卷 / 05期

关键词：

environmental analysis; enzyme biosensor; pesticide; portable;

D O I：

10.1016/j.talanta.2008.01.033

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high sensitive portable biosensor system capable of determining the presence of neurotoxic agents in water has been developed. The system consists of (i) a screen-printed electrode with acetylcholinesterase (AChE) immobilized on it, (ii) a self-developed portable potentiostat with an analog to digital (A/D) converter and a serial interface for transferring data to a portable PC and (iii) an own designed software, developed with Lab-Windows CVI, used to record and process the measurements. The system has been developed to perform high precision amperometrical measurements with low drifts, low noise and a good reproducibility. In the configuration depicted, the percentage of AChE inhibition is proportional to the content of neurotoxic agents in a sample. This type of measurement is performed by the steady-state method from the first steady current (by a phosphate buffer solution) and the second steady current (by an enzymatic reaction produced by the addition of acetylthiocholine chloride to the solution). Validation was performed by analyzing spiked water samples containing pesticides. The design is specially suited for screening purposes, does not need sample preconcentration, is totally autonomous and suitable for the field detection of neurotoxic agents in water. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1208 / 1213

页数：6

共 26 条

[1] Pesticide determination in tap water and juice samples using disposable amperometric biosensors made using thick-film technology [J].

Albareda-Sirvent, M ;

Merkoçi, A ;

Alegret, S .

ANALYTICA CHIMICA ACTA, 2001, 442 (01) :35-44

[2] Detection of organophosphorus insecticides with immobilized acetylcholinesterase - comparative study of two enzyme sensors [J].

Andreescu, S ;

Avramescu, A ;

Bala, C ;

Magearu, V ;

Marty, JL .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 374 (01) :39-45

[3] Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides [J].

Andreescu, S ;

Barthelmebs, L ;

Marty, JL .

ANALYTICA CHIMICA ACTA, 2002, 464 (02) :171-180

[4] A portable fiber-optic pesticide biosensor based on immobilized cholinesterase and sol-gel entrapped bromcresol purple for in-field use [J].

Andreou, VG ;

Clonis, YD .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (1-2) :61-69

[5] Fast, sensitive and cost-effective detection of nerve agents in the gas phase using a portable instrument and an electrochemical biosensor [J].

Arduini, Fabiana ;

Amine, Aziz ;

Moscone, Danila ;

Ricci, Francesco ;

Palleschi, Giuseppe .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 388 (5-6) :1049-1057

[6] Detection of carbamic and organophosphorous pesticides in water samples using a cholinesterase biosensor based on Prussian Blue-modified screen-printed electrode [J].

Arduini, Fabiana ;

Ricci, Francesco ;

Tuta, Catalin S. ;

Moscone, Danila ;

Amine, Aziz ;

Palleschi, Giuseppe .

ANALYTICA CHIMICA ACTA, 2006, 580 (02) :155-162

[7] An update of EU legislation (Directives and Regulations) on food-related issues (safety, hygiene, packaging, technology, GMOs, additives, radiation, labelling): presentation and comments [J].

Arvanitoyannis, IS ;

Choreftaki, S ;

Tserkezou, P .

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2005, 40 (10) :1021-1112

[8] Biosensors designed for environmental and food quality control based on screen-printed graphite electrodes with different configurations [J].

Avramescu, A ;

Andreescu, S ;

Noguer, T ;

Bala, C ;

Andreescu, D ;

Marty, JL .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 374 (01) :25-32

[9] Continuous solid-phase extraction and gas chromatographic determination of organophosphorus pesticides in natural an drinking waters [J].

Ballesteros, E ;

Parrado, MJ .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1029 (1-2) :267-273

[10] Design of a low-cost portable potentiostat for amperometric biosensor [J].

Blanco, J. R. ;

Ferrero, F. J. ;

Campo, J. C. ;

Anton, J. C. ;

Pingarron, J. M. ;

Reviejo, A. J. ;

Manso, J. .

2006 IEEE INSTRUMENTATION AND MEASUREMENT TECHNOLOGY CONFERENCE PROCEEDINGS, VOLS 1-5, 2006, :690-+

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