Absence of microdeletions in the Y chromosome in patients with a history of cryptorchidism and azoospermia or oligospermia

被引:19
作者
Fagerli, J
Schneck, FX
Lee, PA
Bellinger, MF
Witchel, SF
机构
[1] Univ Pittsburgh, Div Endocrinol, Childrens Hosp Pittsburgh, Sch Med, Pittsburgh, PA 15213 USA
[2] Univ Pittsburgh, Childrens Hosp Pittsburgh, Sch Med, Dept Pediat,Div Pediat Endocrinol, Pittsburgh, PA 15213 USA
[3] Univ Pittsburgh, Childrens Hosp Pittsburgh, Sch Med, Div Urol Surg, Pittsburgh, PA 15213 USA
关键词
cryptorchidism; infertility; Y chromosome;
D O I
10.1016/S0015-0282(98)00535-4
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To determine if cryptorchidism is associated with microdeletions of interval 6 of the Y chromosome, we evaluated this locus in men with a history of cryptorchidism with and without azoospermia or oligospermia and in a control group. Design: Clinical study. Setting: Academic research environment. Patient(s): Men in whom surgical treatment of cryptorchidism had been performed in childhood and healthy control male subjects. Intervention(s): None. Main Outcome Measure(s): Genotyping of interval 6 of the Y chromosome. Result(s): Analysis of semen obtained from men treated for cryptorchidism in childhood showed azoospermia or oligospermia in 14 of 38 (37%) men. No microdeletions were identified with polymerase chain reaction amplification of 17 distinct sequence tagged sites located on the long arm of the Y chromosome and the sex determining region on Y (SRY) gene. Conclusion(s): Microdeletions of interval 6 of the Y chromosome were not detected in either the formerly cryptorchid or in the healthy subjects. Although we cannot exclude the possibility of point mutations, we conclude that cryptorchidism or cryptorchidism associated with azoospermia or oligospermia is not due to microdeletions involving interval 6 of the Y chromosome. (C) 1999 by American Society for Reproductive Medicine.
引用
收藏
页码:697 / 700
页数:4
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