B cells productively engage soluble antigen-pulsed dendritic cells: Visualization of live-cell dynamics of B cell dendritic cell interactions

被引：45

作者：

Huang, NN ^{[1
]}

Han, SB ^{[1
]}

Hwang, IY ^{[1
]}

Kehrl, JH ^{[1
]}

机构：

[1] NIAID, Cell Mol Immunol Sect B, Immunoregulat Lab, Bethesda, MD 20892 USA

来源：

JOURNAL OF IMMUNOLOGY | 2005年 / 175卷 / 11期

关键词：

D O I：

10.4049/jimmunol.175.11.7125

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Interactions between B lymphocytes and Ag-bearing dendritic cells (DC) likely occur at inflammatory sites and within lymphoid organs. To better understand these interactions we imaged B cells (TgB) from hen egg lysozyme (HEL) transgenic mice and DC pulsed with HEL (DC-HEL) in collagen matrices. Analysis of live-cell dynamics revealed autonomous movements and repeated encounters between TgB cells and DC-HEL that are best described by a "kiss-run and engage" model, whereas control B cells had only short-lived interactions. Ag localized at contact sites between TgB cells and DC-HEL, and both cell types rearranged their actin cytoskeletons toward the contact zone. The interaction of a TgB cell with a HEL-bearing DC triggered strong Ca2+ transients in the B cells. Thus, B cells can productively interact with DC displaying their cognate Ag.

引用

页码：7125 / 7134

页数：10

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