Food supplementation with milk fermented by Lactobacillus casei DN-114 001 protects suckling rats from rotavirus-associated diarrhea

被引:46
作者
Guérin-Danan, C
Meslin, JC
Chambard, A
Charpilienne, A
Relano, P
Bouley, C
Cohen, J
Andrieux, C [1 ]
机构
[1] INRA, UEPSD, MBS, F-78352 Jouy En Josas, France
[2] INRA, VIM, F-78352 Jouy En Josas, France
[3] VITAPOLE, Danone, F-92350 Le Plessis Robinson, France
关键词
Lactobacillus; fermented milk; rotavirus; suckling rats; diarrhea; intestine;
D O I
10.1093/jn/131.1.111
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Group A rotavirus is the leading cause of diarrhea among children aged 3-36 mo worldwide. Introducing fermented milk products into the infant diet has been proposed for the prevention or treatment of rotavirus diarrhea. The preventive effect of milk fermented by the Lactobacillus casei strain DN-114 001 was studied in a model of germfree suckling rats supplemented daily from d 2 of life and infected with SA11 rotavirus at d 5 (RF group). One group was supplemented with nonfermented milk (RM) and two uninfected groups (CM and CF) received either nonfermented or fermented milk. Frequency and severity of diarrhea were observed. Rats were killed at various times from 0 to 120 h postinfection (p.i.). Bacteria were measured in the intestine, and rotavirus antigens were detected by ELISA in fecal samples and in different parts of the intestine. Histologic observations were made, including vacuolation, morphology of intestinal villi and number of mucin cells. RM rats had diarrhea for 6 d; compared with the CM group, they had alterations of the intestinal mucosa characterized by cellular vacuolation 48 and 72 h p.i. and a lower number of sulfated mucin cells 72 and 96 h p.i. (P < 0.05). Early supplementation with fermented milk significantly decreased the clinical signs of diarrhea from 24 to 144 h p.i. (P < 0.05) and prevented rotavirus infection in all sections of the intestine. Histologic lesions of the small intestine were greatly reduced (P < 0.05) and the number of mucin cells remained unchanged. The data are discussed with respect to the possibility of reducing rotavirus diarrhea in young children by consumption of fermented milk.
引用
收藏
页码:111 / 117
页数:7
相关论文
共 47 条
  • [11] de Roos NM, 2000, AM J CLIN NUTR, V71, P405
  • [12] EFFECTIVENESS OF BIFIDOBACTERIUM-BIFIDUM IN MEDIATING THE CLINICAL COURSE OF MURINE ROTAVIRUS DIARRHEAL
    DUFFY, LC
    ZIELEZNY, MA
    RIEPENHOFFTALTY, M
    DRYJA, D
    SAYAHTAHERIALTAIE, S
    GRIFFITHS, E
    RUFFIN, D
    BARRETT, H
    ROSSMAN, J
    OGRA, PL
    [J]. PEDIATRIC RESEARCH, 1994, 35 (06) : 690 - 695
  • [13] New lessons for rotavirus vaccines
    Glass, RI
    Gentsch, JR
    Ivanoff, B
    [J]. SCIENCE, 1996, 272 (5258) : 46 - 48
  • [14] Lactobacillus GG administered in oral rehydration solution to children with acute diarrhea:: A multicenter European trial
    Guandalini, S
    Pensabene, L
    Abu Zikri, M
    Dias, JA
    Casali, LG
    Hoekstra, H
    Kolacek, S
    Massar, K
    Micetic-Turk, D
    Papadopoulou, A
    de Sousa, JS
    Sandhu, B
    Szajewska, H
    Weizman, Z
    [J]. JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 2000, 30 (01) : 54 - 60
  • [15] Oral bacterial therapy reduces the duration of symptoms and of viral excretion in children with mild diarrhea
    Guarino, A
    Canani, RB
    Spagnuolo, MI
    Albano, F
    DiBenedetto, L
    [J]. JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1997, 25 (05) : 516 - 519
  • [16] Development of a heterologous model in germfree suckling rats for studies of rotavirus diarrhea
    Guerin-Danan, C
    Meslin, JC
    Lambre, F
    Charpilienne, A
    Serezat, M
    Bouley, C
    Cohen, J
    Andrieux, C
    [J]. JOURNAL OF VIROLOGY, 1998, 72 (11) : 9298 - 9302
  • [17] Milk fermented with yogurt cultures and Lactobacillus casei compared with yogurt and gelled milk:: influence on intestinal microflora in healthy infants
    Guerin-Danan, C
    Chabanet, C
    Pedone, C
    Popot, F
    Vaissade, P
    Bouley, C
    Szylit, O
    Andrieux, C
    [J]. AMERICAN JOURNAL OF CLINICAL NUTRITION, 1998, 67 (01) : 111 - 117
  • [18] Pattern of metabolism and composition of the fecal microflora in infants 10 to 18 months old from day care centers
    GuerinDanan, C
    Andrieux, C
    Popot, F
    Charpilienne, A
    Vaissade, P
    Gaudichon, C
    Pedone, C
    Bouley, C
    Szylit, O
    [J]. JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1997, 25 (03) : 281 - 289
  • [19] GUERINDANAN C, 1998, CAH NUTR DIET, V33, P141
  • [20] INTESTINAL-ABSORPTION OF MACROMOLECULES DURING VIRAL-ENTERITIS - AN EXPERIMENTAL-STUDY ON ROTAVIRUS-INFECTED CONVENTIONAL AND GERM-FREE MICE
    HEYMAN, M
    CORTHIER, G
    PETIT, A
    MESLIN, JC
    MOREAU, C
    DESJEUX, JF
    [J]. PEDIATRIC RESEARCH, 1987, 22 (01) : 72 - 78